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NCI-H508 人结肠直肠腺癌

简要描述:NCI-H508 人结肠直肠腺癌,ATCC 细胞|细胞系|细胞株|肿瘤细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件!

  • 产品型号:CCL-253
  • 厂商性质:生产厂家
  • 更新时间:2022-09-06
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NCI-H508 人结肠直肠腺癌

ATCC® Number:CCL-253™Price:
Designations:NCI-H508 [H508]

Depositors:AF Gazdar

Biosafety Level:1

Shipped:frozen

Medium & Serum:

See Propagation



Growth Properties:floating aggregates of round cells with some attached cells

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: cecum
Disease: colorectal adenocarcinoma


Cellular Products:carcinoembryonic antigen (CEA), 1709 ng/ml per 10 exp6 cells per 10 days

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
NCI-H508 人结肠直肠腺癌

Tumorigenic:Yes

Antigen Expression:Blood type A; Rh+; CA19-9 antigen

DNA Profile (STR):Amelogenin: X

CSF1PO: 11,12

D13S317: 8,12

D16S539: 12

D5S818: 12

D7S820: 8,12

THO1: 9,9.3

TPOX: 8,11

vWA: 15,16



Cytogenetic Analysis:modal number = 102; range = 71 to 131; most cells have DM's

Isoenzymes:AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 2

Me-2, 1

PGM1, 1

PGM3, 1



Age:55 years

Gender:male

Ethnicity:Caucasian

Comments:This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil.

The cells contain Dopa decarboxylase, CA19-9 antigen and CEA but do not express the TAG-72 antigen.



Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing:S

Preservation:Culture medium, 95%; DMSO, 5%

References:

23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249













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