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NCI-H508 人结肠直肠腺癌

  • 更新时间:  2020-09-10
  • 产品型号:  CCL-253
  • 简单描述
  • NCI-H508 人结肠直肠腺癌,ATCC 细胞|细胞系|细胞株|肿瘤细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!
详细介绍

NCI-H508 人结肠直肠腺癌

ATCC® Number:CCL-253™Price:
Designations:NCI-H508 [H508]
Depositors:AF Gazdar
Biosafety Level:1
Shipped:frozen
Medium & Serum:

See Propagation             

 

Growth Properties:floating aggregates of round cells with some attached cells
Organism:Homo sapiens (human)
Morphology:epithelial
 
Source:Organ: cecum
Disease: colorectal adenocarcinoma
Cellular Products:carcinoembryonic antigen (CEA), 1709 ng/ml per 10 exp6 cells per 10 days
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
NCI-H508 人结肠直肠腺癌
Tumorigenic:Yes        
Antigen Expression:Blood type A; Rh+; CA19-9 antigen
DNA Profile (STR):Amelogenin: X
CSF1PO: 11,12
D13S317: 8,12
D16S539: 12
D5S818: 12
D7S820: 8,12
THO1: 9,9.3
TPOX: 8,11
vWA: 15,16
Cytogenetic Analysis:modal number = 102; range = 71 to 131; most cells have DM's
Isoenzymes:AK-1, 1
ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 1
Age:55 years
Gender:male
Ethnicity:Caucasian             
Comments:This line was derived from a metastasis to the abdominal wall obtained from a patient after treatment with 5-fluorouracil.
The cells contain Dopa decarboxylase, CA19-9 antigen and CEA but do not express the TAG-72 antigen.
Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:S
Preservation:Culture medium, 95%; DMSO, 5%
References:

23066: Park JG, et al. Characteristics of cell lines established from human colorectal carcinoma. Cancer Res. 47: 6710-6718, 1987. PubMed: 3479249       

 

 


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