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Isolation:
Isolation date: 1982
Tumorigenic:
Yes
DNA Profile （STR）:
Amelogenin: X
CSF1PO: 10,12
D13S317: 10,11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9.3
TPOX: 8
vWA: 14,17
CRL-2095 CAL 27 人舌鳞癌细胞
Cytogenetic Analysis:
aneuploid; modal number = 43
Age:
56 years
Gender:
male
Ethnicity:
Caucasian
Comments:
CAL 27 cells were resistant to treatment with VDS （vindesine sulfate）, CDP （cis-platinum） or ACTD （actinomycin D）.
A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.
Propagation:
ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. Temperature: 37.0°C
Recommended medium （without the additional supplements or serum described under ATCC Medium）:ATCC 30-2002
recommended serum:ATCC 30-2020
References:
23521: Gioanni J, et al. Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment. Eur. J. Cancer Clin. Oncol. 24: 1445-1455, 1988. PubMed: 3181269
