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CRL-2095 CAL 27 人舌鳞癌细胞

简要描述:CRL-2095 CAL 27 人舌鳞癌细胞,ATCC 细胞|细胞系|细胞株|细胞,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件。

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CRL-2095 CAL 27 人舌鳞癌细胞

ATCC® Number:CRL-2095™Price:
Designations:CAL 27

Depositors:C Cardona

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: tongue
Disease: squamous cell carcinoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Isolation:Isolation date: 1982

Tumorigenic:Yes

DNA Profile (STR):

Amelogenin: X
CSF1PO: 10,12
D13S317: 10,11
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9.3
TPOX: 8
vWA: 14,17

CRL-2095 CAL 27 人舌鳞癌细胞



Cytogenetic Analysis:aneuploid; modal number = 43

Age:56 years

Gender:male

Ethnicity:Caucasian

Comments:
CAL 27 cells were resistant to treatment with VDS (vindesine sulfate), CDP (cis-platinum) or ACTD (actinomycin D).
A culture submitted to the ATCC in December 1993 was found to be contaminated with mycoplasma. Progeny were cured by a 21-day treatment with BM Cycline.


Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C


Subculturin


Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature


Doubling Time:35 hrs

Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020


References:

23521: Gioanni J, et al. Two new human tumor cell lines derived from squamous cell carcinomas of the tongue: establishment, characterization and response to cytotoxic treatment. Eur. J. Cancer Clin. Oncol. 24: 1445-1455, 1988. PubMed: 3181269






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