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M1 小鼠白血病细胞

简要描述:TIB-192 M1 小鼠白血病细胞
ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,
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  • 产品型号:TIB-192
  • 厂商性质:生产厂家
  • 更新时间:2021-09-12
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TIB-192 M1 小鼠白血病细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献优培养条件!

TIB-192 M1 小鼠白血病细胞

ATCC® Number:TIB-192™  Price:$355.00
Designations:M1

Depositors:P Ralph

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:suspension

Organism:Mus musculus (mouse)

Morphology:


Source:Disease: myeloid leukemia
Strain: SL
Cell Type: myeloblast;


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
TIB-192

Comments:Established in vitro from spontaneous myeloid leukemia of SL strain mice.
Can be induced to become macrophage-like by a variety of stimulating agents including dexamethasone, LPS and WEHI-3 conditioned medium.
Tested and found negative for ectromelia virus (mousepox).


Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Subculturing:Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 x 10 exp5 cells/ml and maintain between 1 x 10 exp5 and 1 x 10 exp6 cells/ml.


References:1220: Ralph P, et al. Expression and induction in vitro of macrophage differentiation antigens on murine cell lines. J. Immunol. 130: 108-114, 1983. PubMed: 6847876
22710: Ichikawa Y. Differentiation of a cell line of myeloid leukemia. J. Cell. Physiol. 74: 223-234, 1969. PubMed: 4187945
33167: Chen H, et al. Octamer binding factors and their coactivator can activate the murine PU.1 (spi-1) promoter. J. Biol. Chem. 271: 15743-15752, 1996. PubMed: 8663022




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