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H9c2(2-1)大鼠心肌细胞的介绍

发布时间:2014-05-28      点击次数:2083

该细胞由Kimes B和Brandt B从BD1X大鼠胚胎心脏组织的克隆细胞株亚克隆得到;表现出许多骨骼肌的特性。这个细胞株中的成肌细胞能融合形成多核的肌管,并对乙酰胆碱的刺激发生反应。如果培养基中的血清浓度下降到1%,融合很快发生。
动物种别:大鼠。组织来源:心脏,心肌层。形态:成肌细胞
DMEM高糖培养基(GIBCO,货号11995065)+10%进口胎牛血清。
冻存条件:培养液+10%DMSO

H9c2(2-1)大鼠心肌细胞的介绍
以下是此细胞ATCC介绍
 

CRL-1446 H9c2(2-1) 大鼠心肌细胞 的详细介绍
H9c2(2-1) 大鼠心肌细胞

ATCC® Number:  CRL-1446™       
Designations:  H9c2(2-1)  
Depositors:   W Carlisle  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Rattus norvegicus (rat)  
Morphology: myoblast

 
Source: Strain: BD1X 
Organ: heart 
Tissue: myocardium 
Cellular Products: myokinase; creatine phosphokinase; myosin  
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 
Applications: transfection host (Roche FuGENE® Transfection Reagents) 
Receptors: acetylcholine, expressed 
Age:  embryo  
Comments: H9c2(2-1) is a subclone of the original clonal cell line derived from embryonic BD1X rat heart tissue by B. Kimes and B. Brandt and exhibits many of the properties of skeletal muscle. 
Myoblastic cells in this line will fuse to form multinucleated myotubes and respond to acetylcholine stimulation. 
Fusion occurs faster if the serum concentration in the medium is reduced to one percent. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C ​H9c2(2-1) 大鼠心肌细胞
Subculturing:  Protocol: The myoblastic population will become depleted rapidly if the cultures are allowed to become confluent.
To prevent loss of myoblastic cells, cultures should be subcultured before they become confluent, and the line should be recloned periodically with selection for myoblastic cells.

Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended 
Medium Renewal: Every 2 to 3 days 
Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2002
recommended serum:ATCC 30-2020   ​H9c2(2-1) 大鼠心肌细胞
References: 1062: Kimes BW, Brandt BL. Properties of a clonal muscle cell line from rat heart. Exp. Cell Res. 98: 367-381, 1976. PubMed: 943302
32970: Levy AP, et al. Post-transcriptional regulation of vascular endothelial growth factor by hypoxia. J. Biol. Chem. 271: 2746-2753, 1996. PubMed: 8576250 


 

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