咨询热线

15000266580

当前位置:首页  >  技术文章  >  TOV-21G 人卵巢癌细胞

TOV-21G 人卵巢癌细胞

发布时间:2016-08-01      点击次数:1076
Designations:TOV-21G
Depositors: University of Montreal
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Homo sapiens deposited as human
Morphology:epithelial
 
Source:Organ: ovary 
Tumor Stage: grade 3, stage III 
Disease: primary malignant adenocarcinoma; clear cell carcinoma
Cellular Products:keratin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Applications:Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.
Tumorigenic:Yes
Oncogene:p53 + (wild type)
DNA Profile (STR):Amelogenin: X 
CSF1PO: 13,15 
D13S317: 11,12 
D16S539: 10,12 
D5S818: 12,13 
D7S820: 12 
THO1: 7,9.3 
TPOX: 8,11 
vWA: 17
Cytogenetic Analysis:47, XX, +10 [49408 ]
Age:62 years
Gender:female
Comments:This cell line was initiated in October of 1991 from a patient of French-Canadian descent with no family history of ovarian cancer. 
Like OV-90 (ATCC CRL-11732 ), the OV-21G (ATCC CRL-11730 ) cell line exhibits a deletion at chromosome 3p24. The TOV-112D (ATCC CRL-11731 ) cell line does not exhibit a deletion at chromosome 3p24.
Propagation:ATCC complete growth medium: The base medium for this cell line is a 1:1 mixture of MCDB 105 medium containing a final concentration of 1.5 g/L sodium bicarbonate and Medium 199 containing a final concentration of 2.2 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 15%

  • Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
    Temperature: 37.0°C
Subculturing:Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.05% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37?C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37?C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:4 is recommended 
Medium Renewal: Every 3 to 4 days
Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase
Related Products:recommended serum:ATCC 30-2020
References:

42090: Mes-Masson AM, Provencher D. Primary cultures of normal and tumoral human ovarian epithelium. US Patent 5,710,038 dated Jan 20 1998
49408: Provencher DM, et al. Characterization of four novel epithelial cancer cell lines. In Vitro Cell. Dev. Biol. Anim. 36: 357-361, 2000. PubMed: 10949993

CoC1人卵巢癌细胞系Lec1仓鼠卵巢细胞
A2780人卵巢癌细胞OVAC卵巢癌细胞
Anglne人卵巢癌细胞系NIH:OVCAR-3人卵巢癌细胞
Caov-3人乳突状卵巢腺癌细胞PA-1人卵巢畸胎瘤细胞
CHO中国仓鼠卵巢细胞Pr-HNV8菜青虫卵巢细胞
CHO/dhFr-仓鼠卵巢细胞,二氢叶酸还原酶缺陷RP70-a-CHO-0.8MTX中国仓鼠卵巢细胞
CHO-K1中国仓鼠卵巢细胞SF9晚秋粒虫卵巢细胞
CHO-pt转血小板基因仓鼠卵巢细胞SKOV3人卵巢癌细胞
CHO-S中国仓鼠卵巢悬浮细胞SKOV-3/DDP卵巢癌顺铂耐药株
CoC1人卵巢癌细胞系SW1910人卵巢癌细胞
CoC2 卵巢癌 SW626人卵巢腺癌细胞
CTLA4 Ig-24中国仓鼠卵巢细胞TOV-21G人卵巢癌
ES-2人卵巢透明细胞癌ZA1转S9基因仓鼠卵巢细胞
HO-8910人卵巢癌细胞ZA6转S9基因仓鼠卵巢细胞
HO-8910PM人高转移卵巢癌细胞 Cho-K1仓鼠卵巢细胞亚株
HOC1卵巢癌细胞SK-OV-3人卵巢癌细胞
Lec1仓鼠卵巢细胞Sf9昆虫卵巢细胞
CHO-K1仓鼠卵巢细胞亚株ES-2人卵巢透明细胞癌
CHO/dhFr-仓鼠卵巢细胞,二氢叶酸还原酶缺陷SK-OV-3人卵巢癌细胞TOV-21G 人卵巢癌细胞TOV-21G 人卵巢
联系方式

邮箱:xiangfbio@163.com

地址:上海市虹口区赤峰路630弄南雁大厦

咨询热线

400-821-8510

(周一至周日9:00- 19:00)

在线咨询
  • 扫一扫 微信咨询

Copyright©2021 上海复祥生物科技有限公司 All Right Reserved    备案号:沪ICP备10013034号-2     sitemap.xml
技术支持:化工仪器网    管理登陆