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SH-2 鼠杂交瘤细胞

简要描述:HB-10743 SH-2 鼠杂交瘤细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞株|细胞|贴壁细胞|悬浮细胞|;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件

  • 产品型号:HB-10743
  • 厂商性质:生产厂家
  • 更新时间:2021-09-12
  • 访  问  量:247

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HB-10743 SH-2 鼠杂交瘤细胞的详细介绍

HB-10743 SH-2 鼠杂交瘤细胞

ATCC® Number:HB-10743™  Price:$399.00
Designations:SH2

Depositors:Osiris Therapeutics, Inc.

Isotype:IgG1

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:suspension

Organism:Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))

Morphology:lymphoblast


Source:Cell Type: hybridoma: B lymphocyte;

Cellular Products:immunoglobulin; monoclonal antibody; against human mesenchymal stem cells

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HB-10743

Tumorigenic:Yes

Comments:Animals were immunized with human mesenchymal stem cells.
Spleen cells were fused with Sp2/0-Ag14 mouse myeloma cells.
The antibody with mesenchymal stem cells from bone marrow.


Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 20%

  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere ATCC tested fetal bovine serum is available as ATCC Catalog No. 30-2020.
    Temperature: 37.0°C



Subculturing:Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 2 X 10 exp5 cells/ml and maintain between 2 X 10 exp5 and 1 X 10 exp6 cells/ml.


Preservation:culture medium 95%; DMSO, 5%

Related Products:recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X


References:22086: Caplan AI, Haynesworth SE. Human mesenchymal stem cells. US Patent 5,486,359 dated Jan 23 1996
49752: Haynesworth SE, et al. Cell surface antigens on human marrow-derived mesenchymal cells are detected by monoclonal antibodies. Bone 13: 69-80, 1992. PubMed: 1316137




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