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您现在的位置:首页 > 产品中心 > 细胞库 > 人肿瘤细胞、癌细胞 > CRL-9068NCI-H929细胞, 人骨髓瘤细胞株

NCI-H929细胞, 人骨髓瘤细胞株

  • 更新时间:  2020-09-12
  • 产品型号:  CRL-9068
  • 简单描述
  • NCI-H929细胞, 人骨髓瘤细胞株
    原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!
详细介绍

 NCI-H929细胞, 人骨髓瘤细胞株

ATCC® Number:CRL-9068™    Price:$309.00
Designations:NCI-H929 [H929]
Depositors: National Cancer Institute
Isotype:IgA kappa (Recent tests for IgA, kappa have not detected the production of IgA; the cells are producing kappa light chain.)
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:suspension
Organism:Homo sapiens (human)
Morphology:lymphoblast
Source:Organ: bone marrow 
Disease: plasmacytoma; myeloma 
Cell Type: B lymphocyte;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 NCI-H929细胞, 人骨髓瘤细胞株
Receptors:transferrin
Antigen Expression:PCA-1; CD38 +
DNA Profile (STR):Amelogenin: X 
CSF1PO: 11 
D13S317: 12 
D16S539: 9,13 
D5S818: 11,12 
D7S820: 10,12 
THO1: 9.3 
TPOX: 8,11 
vWA: 14,15
Cytogenetic Analysis:Near tetraploid. Most copies of chromosome 8 have the 8q+ abnormality.
Age:62 years                         
Gender:female
Ethnicity:Caucasian
Comments:This cell line was established from a malignant effusion in a patient with myeloma. 
The cells are positive for plasma cell antigen 1 (PCA-1), transferrin receptor, CD38 (T10), but are negative for HLA DR, CALLA and markers of early B cell development. 
They are negative for Epstein-Barr virus nuclear antigen (EBNA). 
The cells have a rearrangement of the c-myc proto oncogene and express c-myc RNA. 
There is also an activated ras allele.
Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: 2-mercaptoethanol to a final concentration of 0.05 mM; fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C
Subculturing:Protocol: Cultures can be maintained by the addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 4 X 10(5) viable cells/ml. 
Interval: Maintain cultures at cell concentrations between 5 X 10(5) and 1 X 10(6) viable cells/ml. 
Medium Renewal: 2 to 3 times per week
Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
References:22372: Hollis GF, et al. Complex translocation disrupts c-myc regulation in a human plasma cell myeloma. Mol. Cell. Biol. 8: 124-129, 1988. PubMed:3275865
23349: Gazdar AF, et al. Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. Blood 67: 1542-1549, 1986. PubMed: 2423157
23351: Ernst TJ, et al. Identification of a second transforming gene, rasn, in a human multiple myeloma line with a rearranged c-myc allele. Blood 72: 1163-1167, 1988. PubMed: 3048435

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