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CCRF HSB-2人急性淋巴母细胞型白血病细胞

简要描述:CCRF HSB-2细胞, 人急性淋巴母细胞型白血病细胞 CCRF HSB-2人急性淋巴母细胞型白血病细胞
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  • 产品型号:CCL-120.1
  • 厂商性质:生产厂家
  • 更新时间:2021-05-11
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 CCRF HSB-2细胞, 人急性淋巴母细胞型白血病细胞

ATCC® Number:CCL-120.1™    Price:$338.00
Designations:CCRF-HSB-2 [CCRF HSB-2, HSB-2]
Depositors: GE Foley
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:suspension
Organism:Homo sapiens (human)
Morphology:lymphoblast
 
Source:Organ: peripheral blood 
Disease: acute lymphoblastic leukemia 
Cell Type: T lymphoblast;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
               CCRF HSB-2细胞, 人急性淋巴母细胞型白血病细胞
Tumorigenic:Yes
Antigen Expression:HLA A1, A2, B12, B17, Cw2; CD5 (78%), CD7 (96%)
DNA Profile (STR):Amelogenin: X,Y 
CSF1PO: 10,12 
D13S317: 10,12 
D16S539: 9,13 
D5S818: 11,12 
D7S820: 11,12 
THO1: 9,10 
TPOX: 8 
vWA: 18,19
Isoenzymes:G6PD, B
Age:11.5 years
Gender:male                          CCRF HSB-2细胞, 人急性淋巴母细胞型白血病细胞
Ethnicity:Caucasian
Comments:Derived from the same buffy coat preparation as CCL-120 (CCRF-SB) by serially transplanting into newborn syrian hamsters.
Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Iscove's Modified Dulbecco's Medium, Catalog No. 30-2005. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:Medium Renewal: Every 2 to 3 days 
Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 3 - 5 X 10 exp5 viable cells/ml. 
A maximum of 2 - 3 X 10 exp6 viable cells/ml is obtainable.
Preservation:culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2005
recommended serum:ATCC 30-2020
derived from same individual:ATCC CCL-120
References:24413: . M.D. Anderson Hospital and Tumor Inst. Monograph. 21: ; 1967.
25969: Adams RA, et al. Direct implantation and serial transplantation of human acute lymphoblastic leukemia in hamsters, SB-2. Cancer Res. 28: 1121-1125, 1968. PubMed: 4872716
26035: . . Proc. Am. Assoc. Cancer Res. 8: 1, 1967.

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