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WPE-int 人正常前列腺上皮细胞

  • 更新时间:  2020-10-15
  • 产品型号:  CRL-2888
  • 简单描述
  • CRL-2888 WPE-int 人正常前列腺上皮细胞,原代细胞|细胞系|细胞株|菌种原代细胞、细胞系。细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!
详细介绍

CRL-2888 WPE-int 人正常前列腺上皮细胞
 

ATCC® Number:CRL-2888™    Price:$329.00
Designations:WPE-int
Depositors: MM Webber, EJ Tokar
Biosafety Level:2 [cells contain human HPV-18 viral DNA sequences ]
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Homo sapiens (human)
Morphology:epithelial
Source:Organ: prostate, normal, peripheral zone 
Cell Type: epithelial
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2888 WPE-int 人正常前列腺上皮细胞
Isolation:Isolation date: 2004 [PubMed: 16351690] [92794]
Receptors:androgen receptor, expressed ( [92794] upregulated upon exposure to androgen)
Tumorigenic:NO
Antigen Expression:kallikrein 3 ( KLK3); prostate specific antigen (PSA); expressed (upregulated upon exposure to androgen) [PubMed: 16351690] [92794]
DNA Profile (STR):Amelogenin: X,Y 
CSF1PO: 13 
D13S317: 8,14 
D16S539: 9,11 
D5S818: 12,15 
D7S820: 10,11 
THO1: 8 
TPOX: 8,11 
vWA: 14,18
Age:54
Gender:male
Ethnicity:Caucasian
Comments:WPE-int cells were derived from the RWPE-1 cell line (ATCC CRL-11609) after two consecutive cycles of single cell cloning. To establish the RWPE-1 cell line, epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome. WPE-int cells exhibit features characteristic of an intermediate cell type on the path to luminal cell differentiation. Cells show very low expression of p63 and MMP-2 and high expression of cytokeratin 18 as compared to the WPE-stem cell line (ATCC CRL-2887). They are anchorage-dependent. [PubMed: 16351690]The WPE-stem cell line (ATCC CRL-2887), which expresses several features characteristic of stem/progenitor cells, was also derived from RWPE-1 cells after single cell cloning.
  1. The parental cell line RWPE-1 was screened for CMV, HBV, HCV, HTLV 1, HTLV 2, HIV 1, HIV 2, JCV, and MoMuLV DNA sequences. Cells were also tested for 25 species of mycoplasma and Acholeplasma laidlawii . Cells tested negative for all of the above. (personal communication from depositor).
Propagation:ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium:
  • 0.05 mg/ml BPE - provided with the K-SFM kit
  • 5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium. 
    Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
    Temperature: 37.0°C 
    Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent.

CRL-2888

ATCC® Number: CRL-2888™ Price: $329.00 
Designations: WPE-int 
Depositors: MM Webber, EJ Tokar 
Biosafety Level: 2 [cells contain human HPV-18 viral DNA sequences ] 
Shipped: frozen 
Medium & Serum: See Propagation 
Growth Properties: adherent 
Organism: Homo sapiens (human) 
Morphology: epithelial


Source: Organ: prostate, normal, peripheral zone 
Cell Type: epithelial 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. 

Isolation: Isolation date: 2004 [PubMed: 16351690] [92794] 
Receptors: androgen receptor, expressed ( [92794] upregulated upon exposure to androgen) 
Tumorigenic: NO 
Antigen Expression: kallikrein 3 ( KLK3); prostate specific antigen (PSA); expressed (upregulated upon exposure to androgen) [PubMed: 16351690] [92794] 
DNA Profile (STR): Amelogenin: X,Y 
CSF1PO: 13 
D13S317: 8,14 
D16S539: 9,11 
D5S818: 12,15 
D7S820: 10,11 
THO1: 8 
TPOX: 8,11 
vWA: 14,18 
Age: 54 
Gender: male 
Ethnicity: Caucasian 
Comments: WPE-int cells were derived from the RWPE-1 cell line (ATCC CRL-11609) after two consecutive cycles of single cell cloning. To establish the RWPE-1 cell line, epithelial cells from the peripheral zone of a histologically normal adult human prostate were transfected with a plasmid carrying one copy of the human papilloma virus 18 (HPV-18) genome. WPE-int cells exhibit features characteristic of an intermediate cell type on the path to luminal cell differentiation. Cells show very low expression of p63 and MMP-2 and high expression of cytokeratin 18 as compared to the WPE-stem cell line (ATCC CRL-2887). They are anchorage-dependent. [PubMed: 16351690]The WPE-stem cell line (ATCC CRL-2887), which expresses several features characteristic of stem/progenitor cells, was also derived from RWPE-1 cells after single cell cloning.
The parental cell line RWPE-1 was screened for CMV, HBV, HCV, HTLV 1, HTLV 2, HIV 1, HIV 2, JCV, and MoMuLV DNA sequences. Cells were also tested for 25 species of mycoplasma and Acholeplasma laidlawii . Cells tested negative for all of the above. (personal communication from depositor). 
Propagation: ATCC complete growth medium: The base medium for this cell line is provided by Invitrogen (GIBCO) as part of a kit: Keratinocyte Serum Free Medium (K-SFM), Kit Catalog Number 17005-042. This kit is supplied with each of the two additives required to grow this cell line (bovine pituitary extract (BPE) and human recombinant epidermal growth factor (EGF). To make the complete growth medium, you will need to add the following components to the base medium: 
•0.05 mg/ml BPE - provided with the K-SFM kit
•5 ng/ml EGF - provided with the K-SFM kit. NOTE: Do not filter complete medium. 
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C 
Growth Conditions: Subculture cells before they reach confluence. Do not allow cells to become confluent. 


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