您好,欢迎进入上海复祥生物科技有限公司网站!
全国服务热线:15000266580
上海复祥生物科技有限公司
产品搜索
PRODUCT SEARCH
产品分类
PRODUCT CLASSIFICATION
查看更多 >>
相关文章
RELEVANT ARTICLES
您现在的位置:首页 > 产品中心 > 细胞库 > 人正常细胞 > CRL-27842V6.11 JHU-67 人胚肾细胞

2V6.11 JHU-67 人胚肾细胞

  • 更新时间:  2020-11-09
  • 产品型号:  CRL-2784
  • 简单描述
  • CRL-2784 2V6.11 JHU-67 人胚肾细胞
    ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞,。细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件
详细介绍

CRL-2784 2V6.11 JHU-67 人胚肾细胞
ATCC® Number:  CRL-2784™     
Designations:  2V6.11 [JHU-67]  
Depositors:   G Ketner  
Biosafety Level: 2 [Cells containing Adenovirus viral DNA sequences ]  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens (human)  
Morphology: epithelial

CRL-2784 2V6.11 JHU-67 人胚肾细胞
Source: Organ: kidney 
Cell Type: epithelialtransformed with adenovirus 5 DNA 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 
Restrictions: Part of the Johns Hopkins Special Collection  
Isolation:  Isolation date: 2001 
Age:  fetus  
Comments: This line was derived from the human embryonic kidney line, 293 in 2001. 293 cells were transfected with the plasmid pVgRXR bearing a Zeocin-resistance selectable marker to obtain 293VgRXR cells. This population was subsequently transfected with the plasmid pEKORF6 containing Ad5 E4 ORF 6 that encodes E4 34K. pEKORF6 is a derivative of the plasmid pIndHydro that contains a hygromycin resistance gene. The vectors contain SV40 viral DNA sequences. The 2V6.11 cell line was originally selected in hygromycin-containing medium and cloned by single-colony isolation with a cloning cylinder. The 2V6.11 cells inducibly express the human adenovirus E4, 34kDa protein (E4 ORF6). 2V6.11 cells exhibit little or no constitutive E4 biologic activity. Induction by ponasterone A, an ecdysone analog, however, results in E4 biologic activity and levels of E4 34kDa protein that are detectable by immunoblotting. These cells can be used as a tool to study the biology of the adenoviral E4 oncoprotein. 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C 
Growth Conditions: The depositor recommends periodic hygromycin selection. 
Subculturing:  Protocol: 
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
An inoculum of 5 X 10(3) to 1 X 10(4) viable cells/cm2 is recommended. 
Incubate cultures at 37°C.

Interval: Maintain cultures at a cell concentration between 2 X 10(4) and 2 X 10(5) cells/cm2. 
Subcultivation Ratio: A subcultivation ratio of 1:6 to 1:10 is recommended 
Medium Renewal: Two to three times weekly 
Preservation:  Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase 
Doubling Time:  23 hrs.  
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
source culture:ATCC JHU-67 
 Related Links   
NCBI Entrez Search  
Make a Deposit  
Frequently Asked Questions  
Material Transfer Agreement  
Technical Support  
Related Cell Culture Products  
 CRL-2784


留言框

  • 产品:

  • 留言内容:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 详细地址:

  • 省份:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
Contact Us
  • 联系QQ:316826860/1784382136/1784382138
  • 联系邮箱:xiangfbio@163.com
  • 传真:021-35080520
  • 联系地址:上海市虹口区赤峰路630弄南雁大厦

扫一扫  微信咨询

©2020 上海复祥生物科技有限公司 版权所有  备案号:沪ICP备10013034号-2   技术支持:化工仪器网    GoogleSitemap    总访问量:185896 管理登陆