MRC-5 人胚肺细胞

CCL-171 MRC-5 人胚肺细胞
ATCC® Number:  CCL-171™        
Designations:  MRC-5  
Depositors:   JP Jacobs  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens (human)  
Morphology: fibroblast

 CCL-171 MRC-5 人胚肺细胞
Source: Organ: lung 
Disease: normal 
Cell Type: fibroblast 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 
Isolation:  Isolation date: September, 1966 
Applications: efficacy testing [92587] 
testing [92346] [92389] 
transfection host (Roche FuGENE® Transfection Reagents)
viruscide testing [92579] [92582] 
Virus Susceptibility: poliovirus 1; herpes simplex; vesicular stomatitis (Indiana) 
Reverse Transcript: negative  
DNA Profile (STR): Amelogenin: X,Y 
CSF1PO: 11,12 
D13S317: 11,14 
D16S539: 9,11 
D5S818: 11,12 
D7S820: 10,11 
THO1: 8 
TPOX: 8 
vWA: 15 
Cytogenetic Analysis: Chromosome Frequency Distribution 50 Cells: 2n = 46. This is a normal diploid human cell line with 46,XY karyotype. The modal chromosome number was 46, occurring in 70% of cells. The rate of polyploidy was 3.6%. Both X and Y chromosomes were normal. 
Isoenzymes:  G6PD, B 
Age:  14 weeks gestation  
Gender:  male  
Ethnicity:  Caucasian  

Temperature: 37.0°C 
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Subculturing:  Protocol: 
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

CCL-171