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HTB-38 HT-29 人结肠癌细胞

简要描述:HTB-38 HT-29 人结肠癌细胞 ATCC 细胞 细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件!

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HTB-38 HT-29 人结肠癌细胞
ATCC® Number:  HTB-38™      
Designations:  HT-29  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent
Organism: Homo sapiens (human)  
Morphology: epithelial

Source: Organ: colon
Disease: colorectal adenocarcinoma
Cellular Products: secretory component of IgA; carcinoembryonic antigen (CEA); transforming growth factor beta binding protein; mucin  
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  

Restrictions: The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.  
Isolation:  Isolation date: 1964  
Applications: transfection host (Nucleofection technology from Lonza
Roche FuGENE® Transfection Reagents)
Receptors: human adrenergic alpha2A [23560]
urokinase receptor (u-PAR)
vitamin D (moderate expression)
urokinase receptor (u-PAR); vitamin D (moderate expression)
Virus Susceptibility: human immunodeficiency virus (HIV, LAV)
Tumorigenic: Yes  
Oncogene: myc +; ras +; myb +; fos +; sis +; p53 +; abl -; ros -; src -  
Antigen Expression: Blood Type A; Rh+; HLA A1, A3, B12, B17, Cw5
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 11,12
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 6,9
TPOX: 8,9
vWA: 17,19
Cytogenetic Analysis: modal number = 71; range = 68 to 72.
The stemline chromosome number is hypertriploid with the 2S component occurring at 2.4%. Seventeen marker chromosomes are found in most metaphases, generally in single copy per chromosome. The marker designations are: M1p-(=t(3p-;?) with a deleted short arm), t(7q;?), t(10q;?), i(13q), 19q+a; M6, ?t(8q;9q-), ?Xp, M9, 6q+, t(13;?)a, t(13;?)b, 19q+b, M14, M15, 15p+, and Xq-. Chromosome 13 is nullisomic and chromosomes 8 and 14 are generally monosomic. No Y chromosome was detected by QM band analysis.
Isoenzymes:  AK-1, 1
ES-D, 1
GLO-I, 1-2
Me-2, 1  
PGM1, 1-2
PGM3, 1-2
Age:  44 years adult  
Gender:  female  
Ethnicity:  Caucasian  
Comments: Ultrastructural features reported for HT-29 cells include microvilli, microfilaments, large vacuolated mitochondria with dark granules, smooth and rough endoplasmic reticulum with free ribosomes, lipid droplets, few primary and many secondary lysosomes. The cells express urokinase receptors, but do not have detectable plasminogen activator activity [PubMed ID: 8381394]. HT-29 cells are negative for CD4, but there is cell surface expression of galactose ceramide (a possible alternative receptor for HIV). The line is positive for expression of c-myc, K-ras, H-ras, N-ras, Myb, sis and fos oncogenes. The p53 antigen is overproduced, and there is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution. N-myc oncogene expression was not detected.
There is a G -> A mutation in codon 273 of the p53 gene resulting in an Arg -> His substitution.
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated McCoy's 5a Medium Modified, Catalog No. 30-2007. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing:  Protocol:
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended
Medium Renewal: 2 to 3 times per week
Preservation:  Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2007
recommended serum:ATCC 30-2020
derivative:ATCC CCL-218
purified DNA:ATCC HTB-38D

HTB-38 HT-29 人结肠癌细胞

MLE-12 小鼠肺上皮细胞  
saos-2 人成骨肉瘤细胞
MA104 非洲绿猴胚胎肾上皮细胞
ball-1 细胞
nci-h226 人肺鳞癌细胞
MCA38 小鼠结肠癌细胞
LTPA 小鼠胰腺癌细胞
NCI-H23 人非小细胞肺癌细胞
293.00     人胚肾细胞

293FT    人胚肾细胞-用于慢病毒包装
293TN    人胚肾细胞--用于慢病毒包装
293AV    人胚肾细胞-用于腺病毒包装
293T    人胚肾细胞
208F    大鼠成纤维细胞
786-O    人肾透明细胞腺癌细胞
A172    人脑胶质母细胞瘤细胞
A549    人肺癌细胞 细胞系购买
AGS    人胃癌细胞
BEL7402 人肝癌细胞系
AZ-521    人胃癌细胞
B16F0    小鼠黑色素瘤细胞
Bel-7402    人肝癌细胞
Bel-7404    人肝癌细胞
BGC-823    人胃癌细胞
C6    大鼠脑胶质瘤细胞
SW480    人结肠腺癌细胞
CaCo-2    人结肠癌细胞
Cho-K1    仓鼠卵巢细胞亚株
COS-7    非洲绿猴SV40转化的肾细胞
GES-1    人胃上皮细胞
HCT-8    人回盲肠癌细胞
Hepa1-6    小鼠肝癌细胞
HepG2    人肝癌细胞
hFOB1.19    人永生化成骨细胞
HGC-27    人胃癌细胞
Hs 746T    人胃癌细胞
HT-29    人结肠癌细胞
KB    人口腔表皮样癌细胞
LoVo    人结肠癌细胞
LLC-PK1    猪肾近曲小管上皮细胞
MCF-10A    人正常乳腺上皮细胞
MCF-7    人乳腺癌细胞
MDA-MB-231    人乳腺癌细胞
MGC80-3    人胃癌细胞
MKN-45    人胃癌细胞

NCI-H460    人大细胞肺癌细胞
NIH-3T3    小鼠成纤维细胞
NUGC-3    人胃癌细胞
Raw264-7    小鼠单核细胞白血病细胞
SGC-7901    人胃癌细胞
SMMC-7721    人肝癌细胞
SNU-638    人胃癌细胞
SNU-739    人肝癌细胞
Tca8113    人舌鳞癌细胞    细胞系购买
U251    人脑胶质瘤细胞
U-373MG    人脑神经胶质瘤细胞
U-87 MG    人脑星形胶质母细胞瘤
BT-474    人乳腺导管癌细胞
PANC-1    人胰腺癌细胞
HEK293    人胚肾细胞
Hep3B    人肝癌细胞
HL-7702    人正常肝细胞
Raw-Blue    小鼠单核细胞白血病细胞
WEHI-231    小鼠B淋巴细胞
OPM-2    人骨髓瘤细胞
NCI-H929    人骨髓瘤细胞

U266    人骨髓瘤细胞
EJ    人膀胱癌细胞
HOC    大鼠肝卵圆细胞
RWPE-1    人正常前列腺上皮细胞
CAL 27    人舌鳞癌细胞
Calu-3    人肺腺癌细胞
Eca-109    人食管癌细胞
DLD-1    人结直肠腺癌上皮细胞
5637.00     人膀胱癌细胞
SK-OV-3    人卵巢癌细胞
GBC-SD    人胆囊癌细胞
BEL7402 人肝癌细胞系
SW620    人结肠癌细胞
MRC-5    人胚肺成纤维细胞     细胞系购买
PC9    人肺癌细胞
PC14    人肺癌细胞
BxPC    人原位胰腺腺癌细胞
PK(15)    猪肾上皮细胞
MDCK    狗肾上皮细胞
3D4/21    猪肺泡巨噬细胞  
MHCC-97L    人肝癌细胞(低转移)
MHCC-97H    人肝癌细胞系(高转移)
MiaCaPa-2    人胰腺癌细胞
HN13    口腔鳞状细胞癌细胞
Bend3    小鼠脑微血管内皮细胞
QBC939    人胆管癌细胞
Ishikawa    人子宫内膜癌细胞
A498    人肾癌细胞
WEHI-231    小鼠B淋巴细胞
P9R    小鼠骨髓基质细胞
P9    小鼠骨髓基质细胞
F98    大鼠脑胶质瘤细胞
NCIH1299    人非小细胞肺癌细胞
NCIH1650-M3    人非小细胞肺癌细胞
293LVT    人胚肾细胞--用于慢病毒包装
EC9706    人食管癌细胞
CNE    人鼻咽癌细胞
H322    人非小细胞肺癌细胞
Panc O2    
S8    BEL7402 人肝癌细胞系
R5    细胞系购买
Pig MSC    猪骨髓间质干细胞
HCAEC    人冠状动脉内皮细胞
marc-145    猴胚胎肾上皮细胞
3D4/21    猪肺泡巨噬细胞
PK(15)    猪肾上皮细胞
MADB-106    大鼠乳腺癌细胞
Sf9    昆虫卵巢细胞
HUVEC[HUV-EC-C]    人脐静脉内皮细胞
PC14    人肺癌细胞

PC9    人肺癌细胞
Eca-109    人食管癌细胞
CAL 27    人舌鳞癌细胞
HOC    肝卵圆细胞
EJ    人膀胱癌细胞

U266    人骨髓瘤细胞
293-mTLR5    人胚肾细胞
Raw-Blue    小鼠单核细胞白血病细胞
HL-7702    人正常肝细胞
Hep3B    人肝癌细胞
U-373MG    人脑神经胶质瘤细胞
Tca-8113    人舌鳞癌细胞
SNU-739    人肝癌细胞
SNU-638    人胃癌细胞
NUGC-3    人胃癌细胞
NIH-3T3    小鼠成纤维细胞
MKN-45    人胃癌细胞
LLC-PK1    猪肾近曲小管上皮细胞
Hs 746T    人胃癌细胞
Hepa1-6    小鼠肝癌细胞
HCT-8    人回盲肠癌细胞
BEL7402 人肝癌细胞系
HaCaT    人永生化表皮细胞
GES-1    人胃上皮细胞
B16-F0    小鼠黑色素瘤细胞
AZ-521    人胃癌细胞
786-O    人肾透明细胞腺癌细胞
208F    大鼠成纤维细胞
293TN    人胚肾细胞--用于慢病毒包装
293FT    人胚肾细胞-用于慢病毒包装
GP-293    人胚肾细胞
293-L.P.    人胚肾细胞



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