咨询热线

15000266580

当前位置:首页 >产品中心>细胞库>小鼠正常细胞>CRL-9078PA 317 小鼠成纤维细胞

PA 317 小鼠成纤维细胞

简要描述:CRL-9078 PA 317 小鼠成纤维细胞,
ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|;细胞库管理规范,
提供的细胞株背景清楚,提供参考文献和培养条件!

  • 产品型号:CRL-9078
  • 厂商性质:生产厂家
  • 更新时间:2021-10-20
  • 访  问  量:544

产品分类

Product Category

相关文章

Related Articles

详细介绍

CRL-9078 PA 317 小鼠成纤维细胞 的详细介绍

CRL-9078 PA 317 小鼠成纤维细胞

ATCC® Number:  CRL-9078™

Designations:

PA317  

Depositors:   Fred Hutchinson Cancer Res. Cntr.

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Mus musculus (mouse)

Morphology: fibroblast

Source: Organ: embryo

Strain: NIH/Swiss

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Roche FuGENE® Transfection Reagents)

Packaging line for retroviruses.

Age:  embryo

Comments: Derived from TK- NIH/3T3 cells by cotransfection with packaging construct DNA, pPAM3, carried in pBR322 and herpes simplex virus thymidine kinase (TK) gene carried in pBR322.

The PA317 cell line was derived from TK- NIH/3T3 cells by cotransfection with packaging construct DNA (pPAM3) carried in pBR322 and the herpes simplex virus thymidine kinase (TK) gene carried in pBR322.

Introduction of retroviral vectors into these cells, by infection or by transfection, results in production of retrovirus virions with an amphotropic host range that are capable of infecting cells of many mammalian species.

Virions produced by this line have been used successfully to transfer genes into humans.

The percentage of PA317 cells that are capable of packaging retroviral vectors decreases slowly with continued passaging of the cell line, presumably due to the loss of the transfected DNA used to create the line.


CRL-9078




产品咨询

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
联系方式

邮箱:xiangfbio@163.com

地址:上海市虹口区赤峰路630弄南雁大厦

咨询热线

400-821-8510

(周一至周日9:00- 19:00)

在线咨询
  • 扫一扫 微信咨询

Copyright©2021 上海复祥生物科技有限公司 All Right Reserved    备案号:沪ICP备10013034号-2     sitemap.xml
技术支持:化工仪器网    管理登陆