CCL-226 C3H/10T1/2, Clone 8 小鼠胚胎成纤维细胞 的详细介绍
CCL-226 C3H/10T1/2, Clone 8 小鼠胚胎成纤维细胞
| ATCC® Number: | CCL-226™ | Price: | $269.00 |
| Designations: | C3H/10T1/2, Clone 8 |
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| | Depositors: | C Heidelberger |
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| | Biosafety Level: | 1 |
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| | Medium & Serum: | See Propagation |
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| | Growth Properties: | adherent |
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| | Organism: | Mus musculus （mouse） |
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| | Morphology: | fibroblast
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| | Source: | Strain: C3H
Organ: embryo |
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| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | CCL-226 |
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| | Applications: | transfection host （Roche FuGENE® Transfection Reagents） |
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| | Tumorigenic: | No |
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| | Reverse Transcript: | negative |
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| | Antigen Expression: | H-2k |
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| | Cytogenetic Analysis: | Mouse karyotype with a modal number of 80 chromosomes. |
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| | Age: | embryo |
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| | Comments: | C3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells. [23019] The cells are very sensitive to post confluence inhibition of cell division, do not produce tumors in syngeneic mice, have no background of spontaneous transformation, nor do they contain overt endogenous transforming murine leukemia or sarcoma viruses. [22697] The cells are contact sensitive. There is no detectable background spontaneous transformation. They are highly susceptible to transformation by chemical agents. [1208] Tested and found negative for ectromelia virus （mousepox）. NOTE: THE INOCULATION DENSITY, FEEDING AND HARVESTING SCHEDULES MUST BE FOLLOWED RIGIDLY IF THE LINE IS TO RETAIN ITS ESSENTIAL CHARACTERISTICS. THE BATCH OF SERUM USED FOR GROWTH AND FOR TRANSFORMATION ASSAYS MAY AFFECT BOTH THE MORPHOLOGY OF THIS LINE AND THE RESULTS OBTAINED. Monolayers established and maintained for the standard transformation assay should be free of all foci after 6 weeks. [1208] The donor recommends that the line be used between the 5th and 15th passages only. |
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| | Propagation: | ATCC complete growth medium: The base medium for this cell line is Eagle's Basal medium with 2 mM L-glutamine , 1.5 g/L sodium bicarbonate and Earle's BSS. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C |
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