咨询热线

15000266580

当前位置:首页 >产品中心>细胞库>小鼠正常细胞>CCL-226C3H/10T1/2, Clone 8 小鼠胚胎成纤维细胞

C3H/10T1/2, Clone 8 小鼠胚胎成纤维细胞

简要描述:CCL-226 C3H/10T1/2, Clone 8 小鼠胚胎成纤维细胞,原代细胞|细胞系|细胞株|菌种;
细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件!

  • 产品型号:CCL-226
  • 厂商性质:生产厂家
  • 更新时间:2022-06-22
  • 访  问  量:861

产品分类

Product Category

相关文章

Related Articles

详细介绍

CCL-226 C3H/10T1/2, Clone 8 小鼠胚胎成纤维细胞 的详细介绍

CCL-226 C3H/10T1/2, Clone 8 小鼠胚胎成纤维细胞

ATCC® Number:CCL-226™  Price:$269.00
Designations:C3
Shipped:frozen
H/10T1/2, Clone 8


Depositors:C Heidelberger

Biosafety Level:1

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Mus musculus (mouse)

Morphology:fibroblast
CCL-226 <strong><strong><strong><strong><strong><strong><strong><strong><strong><strong><strong><strong><strong><strong><strong><strong><strong><strong><strong><strong><strong></strong></strong></strong></strong></strong></strong></strong></strong></strong></strong></strong></strong></strong></strong></strong></strong></strong></strong></strong></strong></strong>


Source:Strain: C3H
Organ: embryo


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CCL-226

Applications:transfection host (Roche FuGENE® Transfection Reagents)

Tumorigenic:No

Reverse Transcript:negative

Antigen Expression:H-2k

Cytogenetic Analysis:Mouse karyotype with a modal number of 80 chromosomes.

Age:embryo

Comments:C3H/10T1/2, Clone 8 was isolated by C. Reznikoff, D. Brankow and C. Heidelberger in 1972 from a line of C3H mouse embryo cells. [23019]

The cells are very sensitive to post confluence inhibition of cell division, do not produce tumors in syngeneic mice, have no background of spontaneous transformation, nor do they contain overt endogenous transforming murine leukemia or sarcoma viruses. [22697]

The cells are contact sensitive.

There is no detectable background spontaneous transformation.

They are highly susceptible to transformation by chemical agents. [1208]

Tested and found negative for ectromelia virus (mousepox).

NOTE: THE INOCULATION DENSITY, FEEDING AND HARVESTING SCHEDULES MUST BE FOLLOWED RIGIDLY IF THE LINE IS TO RETAIN ITS ESSENTIAL CHARACTERISTICS.

THE BATCH OF SERUM USED FOR GROWTH AND FOR TRANSFORMATION ASSAYS MAY AFFECT BOTH THE MORPHOLOGY OF THIS LINE AND THE RESULTS OBTAINED.

Monolayers established and maintained for the standard transformation assay should be free of all foci after 6 weeks. [1208]

The donor recommends that the line be used between the 5th and 15th passages only.



Propagation:ATCC complete growth medium: The base medium for this cell line is Eagle's Basal medium with 2 mM L-glutamine , 1.5 g/L sodium bicarbonate and Earle's BSS. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C












产品咨询

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
联系方式

邮箱:xiangfbio@163.com

地址:上海市虹口区赤峰路630弄南雁大厦

咨询热线

400-821-8510

(周一至周日9:00- 19:00)

在线咨询
  • 扫一扫 微信咨询

Copyright©2022 上海复祥生物科技有限公司 All Right Reserved    备案号:沪ICP备10013034号-2     sitemap.xml
技术支持:化工仪器网    管理登陆