CMCC 43501 阴沟肠杆菌
冻干菌种的复苏培养:
1冻干管开封:用浸过75%酒精的脱脂棉擦净冻干管,用火焰加热其顶端,滴少量无菌水至加热顶端使之破裂,用锉刀或镊子敲下已破裂的冻干管顶端。
2菌种恢复培养:用无菌吸管 0.3-0.5毫升适宜到液体培养基,滴入冻干管内轻轻振荡,使冻干菌体溶解呈悬浮状,吸取全部菌体悬浮液,移植于1-2支建议的斜面培养基上,并在建议的温度下培养。
CMCC 43501 阴沟肠杆菌 注意事项
1、微生物菌种应保藏于低温、清洁和干燥的地方,室温放置时间过长会导致菌种衰退;
2、菌种操作应在无菌条件下进行,防止杂菌污染;
3、斜面菌种保藏时间通常为3-6个月,应根据菌种状况及时转接;冻干菌种保藏时间通常为2-15年;
Organism: | Escherichia coli (Migula) Caslani and Chalmers |
Designations: | EDL 931 |
Isolation: | feces, human |
Depositor: | GK Morris |
History: | ATCC <<--GK Morris<<--J.G. Wells |
Biosafety Level: | 2 |
Shipped: | freeze-dried |
Growth Conditions: | ATCC medium18: Trypticase soy agar Temperature: 37.0°C |
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Antigenic Properties: | serotype O157:H7 |
Cross References: | Nucleotide (GenBank) : AF305917Escherichia coli O157:H7 nonfunctional beta-glucuronidase (uidA) gene, complete cds. |
Comments: | Hemorrhagic colitis [9698] Cytotoxic activity [8277] |
References: | 8277: Beery JT, et al. Cytotoxic activity of Escherichia coli O157:H7 culture filtrate on the mouse colon and kidney. Curr. Microbiol. 11: 335-342, 1984. 9698: Riley LW, et al. Hemorrhagic colitis associated with a rare Escherichia coli serotype. N. Engl. J. Med. 308: 681-685, 1983. PubMed: 6338386 |