咨询热线

15000266580

当前位置:首页 >产品中心>细胞库>人乳腺癌细胞>CRL-2713MDA-kb2细胞, 人乳腺细胞

MDA-kb2细胞, 人乳腺细胞

简要描述:MDA-kb2细胞, 人乳腺细胞
ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|。细胞库管理规范,
提供的细胞株背景清楚,提供参考文献和*培养条件!

  • 产品型号:CRL-2713
  • 厂商性质:生产厂家
  • 更新时间:2024-08-28
  • 访  问  量:1862

产品分类

Product Category

相关文章

Related Articles

详细介绍

MDA-kb2细胞, 人乳腺细胞

ATCC® Number:CRL-2713™ Price:
Designations:MDA-kb2

Depositors:VS Wilson

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:epithelial


Source:Organ: mammary gland; breast
Cell Type: epithelial


Cellular Products:luciferase ( [57526] The cell line expresses firefly luciferase under control of the MMTV promoter that contains response elements for both glucocorticoid receptors (GR) and androgen receptors (AR).)

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
MDA-kb2细胞, 人乳腺细胞

Isolation:Isolation date: November 25, 1998

Applications:assay of androgen receptor ( [57526] MDA-kb2 may be used in an in vitro assay to screen androgen agonist and antagonists and to characterize its specificity and sensitivity to endocrine disrupting chemicals.)

Receptors:androgen receptor [57526]

glucocorticoid receptor, positive [57526]



DNA Profile (STR):Amelogenin: X

CSF1PO: 10,12

D13S317: 12

D16S539: 9

D5S818: 11

D7S820: 10

THO1: 6

TPOX: 10

vWA: 17,18



Age:48 years adult

Gender:female

Ethnicity:Caucasian

Comments:Since both glucocorticoid receptor and androgen receptor are present in the MDA-MB-453 cells, and both receptors can act through the MMTV promoter, compounds that act through either androgen receptor or glucocorticoid receptor activate the MMTV luciferase reporter. Androgen receptor agonists such as dihydrotestosterone, and glucocorticoid receptor agonists such as dexamethasone, corticosterone, and aldosterone induce luciferase expression. Responsiveness of the cell line was monitored over time and was stable for more than 80 passages. The cell line is an in vitro tool for research in endocrine activity of the human androgen receptor and glucocorticoid receptor. It can be used to screen chemicals for both androgen receptor and glucocorticoid receptor mediated activities.

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 100%



parental cell line:ATCC HTB-131



References:57526: Wilson VS, et al. A novel cell line, MDA-kb2, that stably expresses an androgen- and glucocorticoid-responsive reporter for the detection of hormone receptor agonists and antagonists. Toxicol. Sci. 66: 69-81, 2002. PubMed: 11861974



















产品咨询

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
联系方式

邮箱:xiangfbio@163.com

地址:上海市虹口区四平路710号7层

咨询热线

400-821-8510

(周一至周日9:00- 19:00)

在线咨询
  • 扫一扫 微信咨询

Copyright©2024 上海复祥生物科技有限公司 All Right Reserved    备案号:沪ICP备10013034号-2     sitemap.xml
技术支持:化工仪器网    管理登陆