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OVCAR-3 人卵巢腺癌细胞的介绍

发布时间:2014-08-22      点击次数:1900

OVCAR-3 人卵巢腺癌细胞的介绍

形态特性:上皮细胞样
生长特性: 贴壁生长
特征特性:OVCAR-3细胞源自一名60岁白人女性卵巢肿瘤组织,由T.C.Hamiltonyu建系于1982年。该细胞带有雌、雄激素受体。对阿霉素,顺氯氨铂,(左旋)苯丙氨酸氮芥有一定抗药性,适用于卵巢癌的抗药性研究。OVCAR-3染色体数量在亚三倍体范围内。
传代方法: 1:3传代,2-3天传一代
支原体检测:阴性
此细胞为我公司走ATCC保藏中心引进,以下是ATCC介绍
NIH:OVCAR-3 [OVCAR3] (ATCC® HTB-161)
 

OrganismHomo sapiens, human
Tissueovary
Cell Typeepithelial
Product Formatfrozen
Morphologyepithelial
Culture Propertiesadherent
Biosafety Level1
Diseaseadenocarcinoma
Age60 years
Genderfemale
EthnicityCaucasian
Applications

This cell line is a suitable transfection host.

NIH:OVCAR-3 is an appropriate model system in which to study drug resistance in ovarian cancer, and the presence of hormone receptors should be useful for the evaluation of hormonal therapy.

Storage Conditions

liquid nitrogen vapor temperature

Derivation

The NIH:OVCAR-3 line was established in 1982 by T.C. Hamilton, et al. from the malignant ascites of a patient with progressive adenocarcinoma of the ovary.

Clinical Data

Caucasian

female

60 years

Receptor ExpressionAndrogen receptor, positive; estrogen receptor, positive; progesterone receptor, positive
TumorigenicYes
Effects

Yes, Forms colonies in soft agar

Yes, in nude mice inoculated subcutaneously with 10(7) cells

(Tumors developed within 21 days at 100% frequency (5/5).)

Comments

Forms colonies in soft agar and has an abnormal karyotype.

Resistant to clinically relevant concentrations of adriamycin, melphalan and cisplatin.

Both cultured cells and xenografts exhibit androgen and estrogen receptors.

Xenograft models have been used to show that treatment with 17 beta estradiol can induce progesterone receptors in this human ovarian carcinoma.

KaryotypeThe cell line is aneuploid human female, with chromosome counts in the sub to near-triploid range. Several normal chromosomes (N11, N13, N14, N15, N16, N17, and N22) are clearly under-represented. Many of these missing chromosomes are represented in the large number of cytogenetically altered chromosomes identified as marker chromosomes. In addition to the marker chromosomes, there are a large number of other structurally abnormal and unassignable chromosomes that are not recognized as markers. Random loss and gain of chromosomes from cell to cell are noted in the exact chromosome counts and in the analysis of the karyotypes.
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