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T/G HA-VSMC 人血管平滑肌细胞

简要描述:CRL-1999 T/G HA-VSMC 人血管平滑肌细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*培养条件!

  • 产品型号:CRL-1999
  • 厂商性质:生产厂家
  • 更新时间:2022-11-05
  • 访  问  量:814

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CRL-1999 T/G HA-VSMC 人血管平滑肌细胞

ATCC® Number:CRL-1999™    Price:$264.00
Designations:T/G HA-VSMC

Depositors: RR Gifford

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Homo sapiens (human)

Morphology:fibroblast
CRL-1999 T/G HA-VSMC 人血管平滑肌细胞


Source:Organ: aorta

Tissue: smooth muscle

Disease: normal



Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Applications:transfection host (technology from amaxa)

Reverse Transcript:N

DNA Profile (STR):Amelogenin: X

CSF1PO: 10,11

D13S317: 11,12

D16S539: 12

D5S818: 12,13

D7S820: 9,10

THO1: 7,8

TPOX: 8

vWA: 16



Cytogenetic Analysis:This is a normal human cell line with a 46,XX karyotype. The modal chromosome number was 46, occurring in 90% of cells. The rate of polyploid cells was 0.8%. No structurally altered chromosomes were detected in 15 metaphases karyotyped. Both X chromosomes appeared normal.

Age:11 months

Gender:female

Ethnicity:Caucasian

HeLa Markers:N

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.05 mg/ml ascorbic acid; 0.01 mg/ml insulin; 0.01 mg/ml transferrin; 10 ng/ml sodium selenite; 0.03 mg/ml Endothelial Cell Growth Supplement (ECGS); fetal bovine serum to a final concentration of 10%, HEPES to a final concentration of 10 mM, TES to a final concentration of 10 mM.

Temperature: 37.0°C



Subculturing:Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, and incubate at 37C for 5 minutes. Add an equal volume of fresh medium, disperse the cells and centrifuge to pellet the cells. Resuspend the cells in fresh medium, aspirate and dispense into new flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended

Medium Renewal: Every 2 to 3 days



Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature



Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004

recommended serum:ATCC 30-2020




CRL-1999













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