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您现在的位置:首页 > 产品中心 > 细胞库 > 人正常细胞 > CRL-1999T/G HA-VSMC 人血管平滑肌细胞

T/G HA-VSMC 人血管平滑肌细胞

  • 更新时间:  2020-09-11
  • 产品型号:  CRL-1999
  • 简单描述
  • CRL-1999 T/G HA-VSMC 人血管平滑肌细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!
详细介绍

CRL-1999 T/G HA-VSMC 人血管平滑肌细胞

ATCC® Number:CRL-1999™    Price:$264.00
Designations:T/G HA-VSMC
Depositors: RR Gifford
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Homo sapiens (human)
Morphology:fibroblast
CRL-1999 T/G HA-VSMC 人血管平滑肌细胞
Source:Organ: aorta 
Tissue: smooth muscle 
Disease: normal
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications:transfection host (technology from amaxa)
Reverse Transcript:N
DNA Profile (STR):Amelogenin: X 
CSF1PO: 10,11 
D13S317: 11,12 
D16S539: 12 
D5S818: 12,13 
D7S820: 9,10 
THO1: 7,8 
TPOX: 8 
vWA: 16
Cytogenetic Analysis:This is a normal human cell line with a 46,XX karyotype. The modal chromosome number was 46, occurring in 90% of cells. The rate of polyploid cells was 0.8%. No structurally altered chromosomes were detected in 15 metaphases karyotyped. Both X chromosomes appeared normal.
Age:11 months
Gender:female
Ethnicity:Caucasian
HeLa Markers:N
Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.05 mg/ml ascorbic acid; 0.01 mg/ml insulin; 0.01 mg/ml transferrin; 10 ng/ml sodium selenite; 0.03 mg/ml Endothelial Cell Growth Supplement (ECGS); fetal bovine serum to a final concentration of 10%, HEPES to a final concentration of 10 mM, TES to a final concentration of 10 mM.
Temperature: 37.0°C
Subculturing:Protocol: Remove medium, add fresh 0.25% trypsin, 0.53 mM EDTA solution, and incubate at 37C for 5 minutes. Add an equal volume of fresh medium, disperse the cells and centrifuge to pellet the cells. Resuspend the cells in fresh medium, aspirate and dispense into new flasks. 
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended 
Medium Renewal: Every 2 to 3 days
Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor temperature
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020

 

CRL-1999


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