您好,欢迎进入上海复祥生物科技有限公司网站!
全国服务热线:15000266580
上海复祥生物科技有限公司
产品搜索
PRODUCT SEARCH
产品分类
PRODUCT CLASSIFICATION
查看更多 >>
相关文章
RELEVANT ARTICLES
您现在的位置:首页 > 产品中心 > 细胞库 > 人正常细胞 > CRL-2099KU812 人外周血嗜碱性白细胞

KU812 人外周血嗜碱性白细胞

  • 更新时间:  2020-09-11
  • 产品型号:  CRL-2099
  • 简单描述
  • CRL-2099 KU812 人外周血嗜碱性白细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,
    提供的细胞株背景清楚,提供参考文献和Z优培养条件!
详细介绍

CRL-2099 KU812 人外周血嗜碱性白细胞

ATCC® Number:CRL-2099™    Price:$329.00
Designations:KU812
Depositors: K Kishi
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:suspension
Organism:Homo sapiens (human)
Morphology:myeloblast
 
Source:Organ: peripheral blood 
Disease: chronic myelogenous leukemia 
Cell Type: basophil;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-2099 KU812 人外周血嗜碱性白细胞
Isolation:Nigata Japan 
Isolation date: August 1, 1981
Antigen Expression:CD3; Homo sapiens 
alanyl (membrane) aminopeptidase (ANPEP, CD13); Homo sapiens
Cytogenetic Analysis:58, XY, t(9;22)(q34;q11), i(17q), +1, +4, +6, +6, +8, +der(9)t(9;22), + der(9)t(9;22), +der(11)q(11), +14, +15, +19, +21, +21, -9 [44220]
Age:38 years adult
Gender:male
Ethnicity:Japanese
Comments:The KU812 cell line was established from the peripheral blood of a patient in blast crisis of chronic myelogenous leukemia. The cells contain at least one Ph1 (Philadelphia) chromosome. The cell line has some characteristics of basophilic leukocytes (Fc receptors, basophilic granules, histamine production), and are negative for lymphoid markers.
Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C
Subculturing:Protocol: Cultures can be maintained by addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 3 X 10(5) viable cells/ml. . Do not allow the cell concentration to exceed 3 x 10(6) cells/ml. 
Medium Renewal: every 2 to 3 days
Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO 
Storage temperature: liquid nitrogen vapor phase
Doubling Time:20 to 30 hours
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020
derivative:ATCC CRL-2100
derivative:ATCC CRL-2101
References:44220: Kishi K. A new leukemia cell line with Philadelphia chromosome characterized as basophil precursors. Leuk. Res. 9: 381-390, 1985. PubMed: 3858609
44221: Okano H, et al. Hemoglobin synthesis of both adult and fetal types in a human CML cell line. J. Biochem. 104: 162-164, 1988. PubMed:2460439
44222: Okamura S, et al. Expression of cytokine genes in hematological malignancies. Nippon Ketsueki Gakkai Zasshi 52: 1423-1432, 1989. PubMed: 2698048
61145: Blom T, et al. Phenotypic characterization of KU812, a cell line identified as an immature human basophilic leukocyte. Eur. J. Immunol. 22: 2025-2032, 1992. PubMed: 1639103

 

CRL-2099 


留言框

  • 产品:

  • 留言内容:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 详细地址:

  • 省份:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
Contact Us
  • 联系QQ:316826860/1784382136/1784382138
  • 联系邮箱:xiangfbio@163.com
  • 传真:021-35080520
  • 联系地址:上海市虹口区赤峰路630弄南雁大厦

扫一扫  微信咨询

©2020 上海复祥生物科技有限公司 版权所有  备案号:沪ICP备10013034号-2   技术支持:化工仪器网    GoogleSitemap    总访问量:178683 管理登陆