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HTB-125 Hs 578Bst 人正常乳腺细胞

  • 更新时间:  2020-09-11
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  • HTB-125 Hs 578Bst 人正常乳腺细胞, ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!
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HTB-125 Hs 578Bst 人正常乳腺细胞

OrganismHomo sapiens, human
Tissuemammary gland/breast
Cell TypeEpithelial, Myoepithelial
Product Formatfrozen
Morphologyfibroblast
Culture Propertiesadherent
Biosafety Level1
Diseasenormal
Age74 years
Genderfemale
EthnicityCaucasian             

HTB-125 Hs 578Bst 人正常乳腺细胞

Storage Conditionsliquid nitrogen vapor phase
Karyotypemodal number . Both X = 46; range = 42 to 48
This is a diploid human cell line with 46,XX karyotype. Polyploidy occurred at 6.9%chromosomes were normal. The chromosome N9 pair was heteromorphic for the centromeric heterochromatin having one with the normal size and the other about twice the size of the normal.
  
Derivation

Hs 578Bst was derived by A.J. Hackett, et al. from normal breast tissue peripheral to an infiltrating ductal carcinoma which was the source for Hs 578T (see ATCC HTB-126).

Hs 578Bst may have been myoepithelial in origin since the cells possessed microfilaments and clusters of pinocytotic vesicles similar to those seen in myoepithelia in vivo.

 

Receptor ExpressionEpidermal growth factor (EGF)          

 

Comments

No desmosomes were observed, estrogen receptor protein was not present, and no endogenous viruses were detected.

A frozen ampule at unknown population doubling (PDL) was received at the ATCC in 1983. Cells had the potential to reach approximay 22 more population doublings before the onset of senescence. See Batch Specific information for PDL of current Distribution freeze.

Complete Growth MediumThe base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water and supplemented with 1.5 g/L sodium bicarbonate. To make the complete growth medium, add the following components to the base medium: 30 ng/ml mouse EGF; fetal bovine serum to a final concentration of 10%. 
 
Subculturing

Volumes used in this protocol are for 75 cm2 flasks; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels. 
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:8 is recommended                 
Medium Renewal: 2 to 3 times per week

 

 

Note: For more information on enzymatic dissociation and subculturing of cell lines consult Chapter 13 in Culture Of Animal Cells: A Manual Of Basic Technique by R. Ian Freshney, 5th edition, published by Wiley-Liss, N.Y., 2005.

CryopreservationComplete growth medium, 95%; DMSO, 5%. Cell culture tested DMSO is available as ATCC Catalog No. 4-X.

 

Culture Conditions

Temperature: 37°C

Atmosphere: Air, 95%; Carbon dioxide (CO2), 5%


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