CRL-1751 WEHI 164 小鼠纤维肉瘤细胞系，ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细|悬胞浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献优培养条件
CRL-1751 WEHI 164 小鼠纤维肉瘤细胞系
| ATCC® Number: | CRL-1751™ | Price: | $355.00 |
| Designations: | WEHI 164 |
|
| | Depositors: | TW LeBien |
|
| | Biosafety Level: | 1 |
|
| | Shipped: | frozen |
|
| | Medium & Serum: | See Propagation |
|
| | Growth Properties: | adherent |
|
| | Organism: | Mus musculus （mouse） |
|
| | Morphology: | fibroblast
|
|
| | Source: | Disease: fibrosarcoma
Strain: BALB/c
Cell Type: methylcholanthrene induced |
|
| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. | | CRL-1751 |
|
| | Comments: | This line was originally established by M. Rollinghoff and N.L. Warner from a fibrosarcoma induced by subcutaneous injections of 3-methylcholanthrene. After pretreatment with actinomycin D, the cells are highly sensitive to human cytotoxic monocytes, to human tumor necrosis factor and to lymphotoxin. They can be used as target cells for short term chromium release cytotoxicity assays. Tested and found negative for ectromelia virus （mousepox）. |
|
| | Propagation: | ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C |
|
| | Subcu |
|
|
|
|
|
|
| | Refe |
|
|
|
