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FOX-NY 小鼠淋巴瘤细胞

简要描述:CRL-1732 FOX-NY 小鼠淋巴瘤细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞株|细胞|贴壁细胞|悬浮细胞|;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件

  • 产品型号:CRL-1732
  • 厂商性质:生产厂家
  • 更新时间:2021-07-11
  • 访  问  量:362

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CRL-1732 FOX-瘤细胞 的详细介绍

CRL-1732 FOX-NY 小鼠淋NY 小鼠淋巴巴瘤细胞

ATCC® Number:CRL-1732™   Price:$438.00
Designations:FOX-NY
Depositors: RT Taggart
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:suspension
Organism:Mus musculus (mouse)
Morphology:lymphoblast
 
Source:Disease: myeloma
Strain: BALB/c
Cell Type: B lymphoblast;
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-1732
Comments:FOX-NY was derived from the NS-1 myeloma cell line.
It is resistant to analogs of both adenosine and guanine (e.g., 2,6-diaminopurine and 8-azaguanine) as a result of deficiencies in hypoxanthine phosphoribosyl transferase (HPRT, HGPRT) and adenosine phosphoribosyl transferase (APRT).
When used as the immortal partner in a fusion using B cells from a female mouse with the Rb(8.12) translocation, there is a positive selection for the immunoglobulin heavy chain locus.
If the fusion products are grown in the presence of aminopterin, adenine and thymidine (or azaserine and adenine).
Selection for the Igh locus results in more stable antibody production in the resulting hybridomas.
Tested and found negative for ectromelia virus (mousepox).
Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C

 

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