您好,欢迎进入上海复祥生物科技有限公司网站!
全国服务热线:15000266580
上海复祥生物科技有限公司
产品搜索
PRODUCT SEARCH
产品分类
PRODUCT CLASSIFICATION
查看更多 >>
相关文章
RELEVANT ARTICLES
您现在的位置:首页 > 产品中心 > 细胞库 > 人肿瘤细胞、癌细胞 > AN3 CA人子宫内膜腺癌细胞

人子宫内膜腺癌细胞

  • 更新时间:  2020-09-04
  • 产品型号:  AN3 CA
  • 简单描述
  • HTB-111 AN3 CA 人子宫内膜腺癌细胞,
    原代细胞|细胞系|细胞株|菌种;细胞库管理规范,
    提供的细胞株背景清楚,提供参考文献和培养条件!
详细介绍

HTB-111 AN3 CA 人子宫内膜腺癌细胞

ATCC® Number: HTB-111™ Price: $399.00
Designations: AN3 CA
Depositors: CJ Dawe
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Proper sapiens (human)

Morphology: epties: adherent
Organism: Homoithelial

HTB-111 AN3 CA 人子宫内膜腺癌细胞
Source: Organ: uterus
Tissue: endometrium
Disease: adenocarcinoma
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Tumorigenic: Yes
DNA Profile (STR): Amelogenin: X
CSF1PO: 12,14,15
D13S317: 12,14
D16S539: 10,14
D5S818: 11,14
D7S820: 7,10,7.1
THO1: 10,9.3
TPOX: 8,10
vWA: 14,20
Isoenzymes: AK-1, 1-2
ES-D, 1
G6PD, B
GLO-I, 2
PGM1, 1
PGM3, 1-2
Age: 55 years
Gender: female
Ethnicity: Caucasian
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol: Volumes used in this protocol are for 75 sq. cm flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes.
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin-0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5.Add appropriate aliquots of the cell suspension to new culture vessels.
6.Incubate cultures at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended
Medium Renewal: 2 to 3 times per week
Preservation: Freeze medium: Culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References: 22517: Dawe CJ, et al. Growth in continuous culture, and in hamsters, of cells from a neoplasma assoicated with Acanthosis nigricans. J. Natl. Cancer Inst. 33: 441-456, 1964. PubMed: 14207855
22539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 327080
29988: Hendricks DT, et al. FHIT gene expression in human ovarian, endometrial, and cervical cancer cell lines. Cancer Res. 57: 2112-2115, 1997. PubMed: 9187105

HTB-111 AN3 CA


留言框

  • 产品:

  • 留言内容:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 详细地址:

  • 省份:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
Contact Us
  • 联系QQ:316826860/1784382136/1784382138
  • 联系邮箱:xiangfbio@163.com
  • 传真:021-35080520
  • 联系地址:上海市虹口区赤峰路630弄南雁大厦

扫一扫  微信咨询

©2020 上海复祥生物科技有限公司 版权所有  备案号:沪ICP备10013034号-2   技术支持:化工仪器网    GoogleSitemap    总访问量:178570 管理登陆