您好,欢迎进入上海复祥生物科技有限公司网站!
全国服务热线:15000266580
上海复祥生物科技有限公司
产品搜索
PRODUCT SEARCH
产品分类
PRODUCT CLASSIFICATION
查看更多 >>
相关文章
RELEVANT ARTICLES
您现在的位置:首页 > 产品中心 > 细胞库 > 人正常细胞 > HUV-EC-C人脐静脉内皮细胞

人脐静脉内皮细胞

  • 更新时间:  2020-09-04
  • 产品型号:  HUV-EC-C
  • 简单描述
  • CRL-1730 HUV-EC-C 人脐静脉内皮细胞,
    ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,
    细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件
详细介绍

CRL-1730 HUV-EC-C 人脐静脉内皮细胞

ATCC® Number: CRL-1730™ Price: $280.00
Designations: HUV-EC-C
Biosafety Level: 1
Shipped: frozen
Medium & Serum: See Propagation
Growth Properties: adherent
Organism: Homo sapiens (human)
Morphology: endothelial

CRL-1730 HUV-EC-C 人脐静脉内皮细胞
Source: Organ: umbilical vein
Tissue: vascular endothelium
Disease: normal
Cell Type: endothelial
Cellular Products: factor VIII [23284]
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (technology from amaxa)
Tumorigenic: No
DNA Profile (STR): Amelogenin: X
CSF1PO: 11,12
D13S317: 9,11
D16S539: 11,12
D5S818: 11,12
D7S820: 8,12
THO1: 6,9.3
TPOX: 8,11
vWA: 16
Cytogenetic Analysis: This is a hypodiploid human cell line. The modal chromosome number was 45 occurring in 72% of cells counted. The rate of polyploid cells was 15.8%. All cells had monosomic N13 and the subclone with additional monosomic N15 predominates. Other coexisting subclones include those with 46,XX,-11,-13,i(11p),i(11q) and 46,XX,+11,-13 karyotypes. Both X chromosomes appear normal.
Comments: Endothelial Cell Growth Supplement (ECGS) and unidentified factors from bovine pituitary, hypothalamus or whole brain extracts are mitogenic for this line. [23284]
The cells have a life expectancy of 50 to 60 population doublings.
Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated of F-12K Medium, Catalog No. 30-2004. To make the complete growth medium, add the following components to the base medium: 0.1 mg/ml heparin; 0.03-0.05 mg/ml endothelial cell growth supplement (ECGS); adjust to a final concentration of 10% fetal bovine serum.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing: Protocol:
1.Remove and discard culture medium.
2.Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
3.Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
4.Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
5.Add appropriate aliquots of the cell suspension to new culture vessels.
6.Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Two to three times per week
Preservation: Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2020
References: 23284: Hoshi H, McKeehan WL. Brain- and liver cell-derived factors are required for growth of human endothelial cells in serum-free culture. Proc. Natl. Acad. Sci. USA 81: 6413-6417, 1984. PubMed: 6333682
29192: Zahedi K. Characterization of the binding of serum amyloid P to laminin. J. Biol. Chem. 272: 2143-2148, 1997. PubMed: 8999915
33021: Soker S, et al. Characterization of novel vascular endothelial growth factor (VEGF) receptors on tumor cells that bind VEGF165 via its exon 7-endoded domain. J. Biol. Chem. 271: 5761-5767, 1996. PubMed: 8621443

CRL-1730 HUV-EC-C


留言框

  • 产品:

  • 留言内容:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 详细地址:

  • 省份:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
Contact Us
  • 联系QQ:316826860/1784382136/1784382138
  • 联系邮箱:xiangfbio@163.com
  • 传真:021-35080520
  • 联系地址:上海市虹口区赤峰路630弄南雁大厦

扫一扫  微信咨询

©2020 上海复祥生物科技有限公司 版权所有  备案号:沪ICP备10013034号-2   技术支持:化工仪器网    GoogleSitemap    总访问量:177655 管理登陆