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WERI-Rb-1细胞,人视网膜神经胶质瘤细胞

简要描述:WERI-Rb-1细胞,人视网膜神经胶质瘤细胞
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  • 产品型号:HTB-169
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  • 更新时间:2021-09-09
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WERI-Rb-1细胞,人视网膜神经胶质瘤细胞

Designations:WERI-Rb-1

Depositors:TW Sery

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:susopension

Organism:Hom sapiens

Morphology:grape-like clusters of round cells


Source:Organ: eye
Tissue: retina
Disease: retinoblastoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
Isolation:Isolation date: 1974

Applications:transfection host

Tumorigenic:Yes                    WERI-Rb-1细胞,人视网膜神经胶质瘤细胞

Cytogenetic Analysis:This is a near diploid line. The modal chromosome number is 47 occurring at 38%, and the rate of polyploidy is 9%. Fifteen to sixteen marker chromosomes are present in all cells. They are t(1,?), t(3p,5q), der(3)t(?q29;?), t(3q,?), 5q+, i(6p), t(7q,?), 9q+, t(10q,21q), 16q+ and five to six others. Normal chromosomes 3, 10, 13 and 16 are absent. There are two copies of the X chromosome. No Y chromosomes were detected in QM stained preparations

Isoenzymes:ES-D, 1
G6PD, B
GLO-I, 2
Me-2, 1
PGM1, 1
PGM3, 0


Age:1 year

Gender:female

Ethnicity:Caucasian

Comments:The WERI-Rb-1 line is one of two human retinoblastoma cell lines established in 1974 by R.M. McFall and T.W. Sery.
The cells survive culturing in Difco Bacto-Agar but do not form colonies.
Scanning electron microscopy reveals some variation in the number and frequency of surface blebs, lamellipodia and microvilli.
The line is of interest in studies of cell differentiation, animal models of tumor therapy and biochemical evaluations.


Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C


Subculturing:Protocol: Cultures can be maintained by addition of fresh medium or replacement of medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension in fresh medium at 2 - 3 X 10 exp5 viable cells/ml. Maintain cell density between 1 X 10 exp5 and 1 to 2 X 10 exp6 viable cells/ml.
Medium Renewal: Every 3 to 4 days


Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase


Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001
recommended serum:ATCC 30-2020


References:23026: McFall RC, et al. Characterization of a new continuous cell line derived from a human retinoblastoma. Cancer Res. 37: 1003-1010, 1977. PubMed: 844036
23030: McFall RC, et al. Scanning electron microscopic observation of two retinoblastoma cell lines. Cancer Res. 38: 2827-2835, 1978. PubMed:679190
32287: Rostomily RC, et al. Expression of neurogenic basic helix-loop-helix genes in primitive neuroectodermal tumors. Cancer Res. 57: 3526-3531, 1997. PubMed: 9270024





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