咨询热线

15000266580

当前位置:首页 >产品中心>细胞库>人肿瘤细胞、癌细胞>HTB-67SK-MEL-1 细胞,人皮肤黑色素瘤细胞

SK-MEL-1 细胞,人皮肤黑色素瘤细胞

简要描述:SK-MEL-1 细胞,人皮肤黑色素瘤细胞
ATCC 细胞|细胞系|细胞株|细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*培养条件!

  • 产品型号:HTB-67
  • 厂商性质:生产厂家
  • 更新时间:2024-11-16
  • 访  问  量:1412

产品分类

Product Category

相关文章

Related Articles

详细介绍

SK-MEL-1 细胞,人皮肤黑色素瘤细胞

ATCC® Number:HTB-67™ Price:
Designations:SK-MEL-1Depositors:G Trempe, LJ OldBiosafety Level:1Shipped:frozenMedium & Serum:See PropagationGrowth Properties:suspensionOrganism:Homo sapiens  SK-MEL-1 细胞,人皮肤黑色素瘤细胞Source:Organ: skin Disease: malignant melanoma Derived from metastatic site: lymphatic systemCellular Products:melaninPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. Restrictions:The cells are distributed for research purposes only. The Memorial Sloan-Kettering Cancer Center releases the line subject to the following: 1.) The cells or their products must not be distributed to third parties. Commercial interests are the exclusive property of Memorial Sloan-Kettering Cancer Center. 2.) Any proposed commercial use of these cells must first be negotiated with The Director, Office of Industrial Affairs, Memorial Sloan-Kettering Cancer Center, 1275 York Avenue, New York, NY 10021; phone (212) 639-6181; (212) 717-3439.Applications:Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis. Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.Tumorigenic:YesAntigen Expression:Blood Type A; Rh+DNA Profile (STR):Amelogenin: X,Y CSF1PO: 12,13 D13S317: 11 D16S539: 11,12 D5S818: 12,13 D7S820: 12 THO1: 6 TPOX: 11 vWA: 16,17Isoenzymes:AK-1, 1 ES-D, 1 G6PD, B GLO-I, 1-2 PGM1, 1 PGM3, 1Age:29 yearsGender:maleEthnicity:CaucasianComments:F. Oettgen and associates isolated this line using cells obtained from the thoracic duct of a patient with widespread and rapidly progressing malignant melanoma. Electron microscopy revealed pigment granules relating both to synthesis and to phagocytosis. Antibody to this line was detected in 63% of patients with malignant melanoma and in 10% of patients with other diseases.Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.Temperature: 37.0°CSubculturing:Medium Renewal: 2 to 3 times per week Cultures can be maintained by addition or replacement of fresh medium. Establish new cultures at 1 X 10 exp5 cells/ml and maintain at 2 X 10 exp5.Preservation:Culture medium, 95%; DMSO, 5%Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003recommended serum:ATCC 30-2020References:1117: Oettgen HF, et al. Suspension culture of a pigment-producing cell line derived from a human malignant melanoma. J. Natl. Cancer Inst. 41: 827-843, 1968. PubMed: 487957822536: Fogh J, et al. Absence of HeLa cell contamination in 169 cell lines derived from human tumors. J. Natl. Cancer Inst. 58: 209-214, 1977. PubMed: 83387122539: Fogh J, et al. One hundred and twenty-seven cultured human tumor cell lines producing tumors in nude mice. J. Natl. Cancer Inst. 59: 221-226, 1977. PubMed: 32708022816: Giraldo G, et al. Human sarcomas in culture. Foci of altered cells and a common antigen; induction of foci and antigen in human fibroblast cultures by filtrates. J. Exp. Med. 133: 454-478, 1971. PubMed: 432944723256: Carey TE, et al. Cell surface antigens of human malignant melanoma: mixed hemadsorption assays for humoral immunity to cultured autologous melanoma cells. Proc. Natl. Acad. Sci. USA 73: 3278-3282, 1976. PubMed: 106761932488: Geiger T, et al. Antitumor activity of a PKC-alpha antisense oligonucleotide in combination with standard chemotherapeutic agents against various human tumors transplanted into nude mice. Anticancer Drug Des. 13: 35-45, 1998. PubMed: 9474241



















产品咨询

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
联系方式

邮箱:xiangfbio@163.com

地址:上海市虹口区四平路710号7层

咨询热线

400-821-8510

(周一至周日9:00- 19:00)

在线咨询
  • 扫一扫 微信咨询

Copyright©2024 上海复祥生物科技有限公司 All Right Reserved    备案号:沪ICP备10013034号-2     sitemap.xml
技术支持:化工仪器网    管理登陆