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您现在的位置:首页 > 产品中心 > 细胞库 > 人肿瘤细胞、癌细胞 > CCL-138Detroit 562细胞, 人咽头癌胸水转移细胞

Detroit 562细胞, 人咽头癌胸水转移细胞

  • 更新时间:  2020-10-14
  • 产品型号:  CCL-138
  • 简单描述
  • Detroit 562细胞, 人咽头癌胸水转移细胞
    ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件
详细介绍

 Detroit 562细胞, 人咽头癌胸水转移细胞

OrganismHomo sapiens, human
Tissuepharynx: derived from metastatic site: pleural effusion
Product Formatfrozen
Morphologyepithelial
Culture Propertiesadherent
Biosafety Level1
Diseasepharyngeal carcinoma
Age*****
Genderfemale
EthnicityCaucasian
Storage Conditionsliquid nitrogen vapor phase

  Karyotype   Modal number = 64; range = 58 to128
A large subterminal marker chromosome, arm ratio 3:4, is found in 94% of the cells karyotyped. Five to 6 minute chromosomes are present in each cell. Note: Cytogenetic information is based on initial seed stock at ATCC. Cytogenetic instability has been reported in the literature for some cell lines   Clinical Data  

Detroit 562细胞, 人咽头癌胸水转移细胞

Caucasian

female

  Genes Expressed  

keratin

  Virus Susceptibility   Human poliovirus 1 
Vesicular stomatitis virus              
  Comments  

The cells are positive for keratin by immunoperoxidase staining.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%. 
 

Subculturing

 

 

 

 

Volumes used in this protocol are for 75 cm2 flask; proportionally reduce or increase amount of dissociation medium for culture vessels of other sizes. 

  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 mL of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 mL of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.        
  7.  

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended

Medium Renewal: Every 2 to 3 days

Cryopreservation

Freeze Medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage Temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: Air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C              CCL-138 Detroit 562 人咽头癌胸水转移细胞


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