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SNU-449细胞, 人肝癌细胞

简要描述:SNU-449细胞, 人肝癌细胞
ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞,细胞库管理规范,提供 *培养条件

  • 产品型号:CRL-2234
  • 厂商性质:生产厂家
  • 更新时间:2021-09-09
  • 访  问  量:453

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详细介绍

SNU-449细胞, 人肝癌细胞(ATCC® CRL-2234)

Permits and Restrictions

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OrganismHomo sapiens, human
Tissue

liver

Product Formatfrozen
Morphologyepithelial; diffusely spreading cells
Culture Propertiesadherent
Biosafety Level2  [Cells contain Hepatitis B virus]
Diseasegrade II-III/IV,hepatocellular carcinoma
Age52 years
Gendermale
EthnicityAsian
Storage Conditionsliquid nitrogen vapor phase
Karyotypeaneuploid; modal number = 57
Derivation

SNU-449 was derived in 1990 by J.-G. Park and associates from a primary hepatocellular carcinoma taken from a Korean patient prior to cytotoxic therapy.

Tumor cells were initially cultured in ACL-4 medium supplemented with 5% heat inactivated fetal bovine serum. After establishment, cultures were maintained in RPMI 1640 supplemented with 10% heat-inactivated fetal bovine serum.
Clinical Data

52 years

Asian

male

SNU-449细胞, 人肝癌细胞

Comments

Hepatitis B virus (HBV) DNA was detected by Southern blot hybridization. HBV genomic RNA was not expressed.

Grossly, the original tumor was single nodular with perinodular extensions. Histologically, it was predominantly compact and minor trabecular type.

The cultured cells contain a single or double nucleus.

Complete Growth MediumThe base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: heat-inactivated fetal bovine serum to a final concentration of 10%.
SubculturingProtocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:5 to 1:10 is recommended

Medium Renewal: Every 2 to 3 days


Cryopreservation

Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO

Storage temperature: liquid nitrogen vapor phase

Culture Conditions

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37°C



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