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您现在的位置:首页 > 产品中心 > 细胞库 > 人正常细胞 > CRL-8083MC/CAR细胞,人外周血淋巴细胞

MC/CAR细胞,人外周血淋巴细胞

  • 更新时间:  2020-09-11
  • 产品型号:  CRL-8083
  • 简单描述
  • MC/CAR细胞,人外周血淋巴细胞
    ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!
详细介绍

 MC/CAR细胞,人外周血淋巴细胞

ATCC® Number:CRL-8083™    Price:$417.00
Designations:MC/CAR
Depositors: Research Corporation
Isotype:IgG1 (cytoplasmic); kappa light chain
Biosafety Level:2
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:suspension
Organism:Homo sapiens (human)
Morphology:lymphoblast
 
Source:Organ: peripheral blood 
Disease: plasmacytoma; myeloma 
Cell Type: B lymphocyte;
Cellular Products:immunoglobulin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anycone purchasing ATCC material is ultimay responsible for obtaining the permits. Please clik here for information regarding the specific requirements for shipment to your location.
MC/CAR细胞,人外周血淋巴细胞
Receptors:complement (C3b)
Tumorigenic:Yes
Antigen Expression:HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 +
DNA Profile (STR):Amelogenin: X,Y 
CSF1PO: 11,12 
D13S317: 11,13 
D16S539: 11,12 
D5S818: 11,14 
D7S820: 9,13 
THO1: 6,9 
TPOX: 8 
vWA: 17,18
Cytogenetic Analysis:normal male diploid
Isoenzymes:ADA, 1 
AK-1, 1 
ES-D, 1 
G6PD, B 
GLO-I, 1 
PGM1, 1 
PGM3, 1
Age:81 years
Gender:male
Ethnicity:Caucasian
Comments:Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270] 
The cells produce, but do not secrete, immunoglobulin. 
No surface immunoglobulin. 
8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures. 
Can be used as a human fusion line.
Propagation:ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%
Temperature: 37.0°C
Subculturing:Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 1 X 10 exp6 cells/ml.
References:3535: Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984
43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375
43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762
58062: Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719

 

 


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