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您现在的位置:首页 > 产品中心 > 细胞库 > 人正常细胞 > CCL-186IMR-90细胞, 人胚肺成纤维细胞

IMR-90细胞, 人胚肺成纤维细胞

  • 更新时间:  2020-11-08
  • 产品型号:  CCL-186
  • 简单描述
  • IMR-90细胞, 人胚肺成纤维细胞
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详细介绍

IMR-90细胞, 人胚肺成纤维细胞

ATCC® Number:  CCL-186™       
Designations:  IMR-90  
Depositors:   WW Nichols  
Biosafety Level: 1  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent 
Organism: Homo sapiens (human)  
Morphology: fibroblast

 IMR-90细胞, 人胚肺成纤维细胞
Source: Organ: lung 
Disease: normal 
Cell Type: fibroblast 
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
 
Applications: transfection host (Nucleofection technology from Lonza) 
Virus Susceptibility: Human poliovirus 1
Human poliovirus 2
Varicella-Zoster
Herpes simplex virus 1
Herpes simplex virus 2
Human poliovirus 3
Vaccinia virus
Human herpesvirus 5
Vesicular stomatitis virus 
Reverse Transcript: negative  
DNA Profile (STR): Amelogenin: X 
CSF1PO: 11,13 
D13S317: 11,13 
D16S539: 10,13 
D5S818: 12,13 
D7S820: 9,12 
THO1: 8,9.3 
TPOX: 8,9 
vWA: 16,19 
Cytogenetic Analysis: normal human female; diploid; stable 
Isoenzymes:  G6PD, B 
Age:  16 weeks gestation  
Gender:  female  
Ethnicity:  Caucasian  
Comments: The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. [22381] 
The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains. 
The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. [22381] 
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5% 
Temperature: 37.0°C 
Subculturing:  Protocol: 
Remove and discard culture medium.
Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
Add appropriate aliquots of the cell suspension to new culture vessels.
Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended 
Medium Renewal: Every 3 to 4 days 
Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase 
Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020 
References: 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339
32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642
33041: Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564 
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