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您现在的位置:首页 > 产品中心 > 细胞库 > 人正常细胞 > CCL-25WISH 细胞,人羊膜细胞

WISH 细胞,人羊膜细胞

  • 更新时间:  2020-09-11
  • 产品型号:  CCL-25
  • 简单描述
  • WISH 细胞,人羊膜细胞
    原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!
详细介绍

 WISH 细胞,人羊膜细胞

ATCC® Number:CCL-25™    Price:$323.00
Additional information about this cell line
Designations:WISH
Depositors: L Hayflick
Biosafety Level:2
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Homo sapiens (human)
Morphology:epithelial
 
Source:Organ: HeLa contaminant
Cellular Products:keratin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Virus Susceptibility:poliovirus 1, 2; adenovirus 3; vesicular stomatitis (Indiana)
Reverse Transcript:negative
DNA Profile (STR):Amelogenin: X 
CSF1PO: 9,10 
D13S317: 13.3 
D16S539: 9,10 
D5S818: 11,12 
D7S820: 8,12 
THO1: 7 
TPOX: 8,12 
vWA: 16,18
Isoenzymes:G6PD, A
HeLa Markers:Y                

 WISH 细胞,人羊膜细胞

Comments:This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. 
The cells are positive for keratin by immunoperoxidase staining. 
NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contamination
Propagation:ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%
Temperature: 37.0°C
Subculturing:Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended 
Medium Renewal: Twice per week 
Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.
Preservation:culture medium 95%; DMSO, 5%
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
References:22153: Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous cultivation. Exp. Cell Res. 23: 14-20, 1961. PubMed:13712490
26034: . . Am. J. Dis. Child. 103: 460, 1962.

 

 


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