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JeKo-1 人套细胞淋巴瘤细胞

简要描述:CRL-3006 JeKo-1 人套细胞淋巴瘤细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*培养条件!

  • 产品型号:CRL-3006
  • 厂商性质:生产厂家
  • 更新时间:2021-09-09
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CRL-3006 JeKo-1 人套细胞淋巴瘤细胞

ATCC® Number:CRL-3006™    Price:$417.00
Designations:JeKo-1

Depositors:Elias Campo

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:suspension

Organism:Homo sapiens (human)

Morphology:lymphoblast
Characterization Data


Source:Organ: peripheral blood
Tissue: Mantle Cell Lymphoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
CRL-3006 JeKo-1 人套细胞淋巴瘤细胞

Antigen Expression:CD3-, CD5+, CD10+, CD19+ (verified at ATCC )

DNA Profile (STR):Amelogenin:X
CSF1PO:9,12
D13S317:8,9
D16S539:12
D5S818:10,13
D7S820:10,11
THO1:7
TPOX:8
vWA: 14


Age:78

Gender:female

Comments:The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. [PubMed: 9753063]

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C


Subculturing:Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 2.5 X 10(6) cells/ml.

Preservation:Freeze medium: 30% RPMI; 60% FBS; 10% DMSO
Storage temperature: liquid nitrogen vapor phase


References:92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
16172573: Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697
16172575: Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063

CRL-3006

ATCC® Number:CRL-3006™    Price:$417.00
Designations:JeKo-1

Depositors: Elias Campo

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:suspension

Organism:Homo sapiens (human)

Morphology:lymphoblast
Characterization Data


Source:Organ: peripheral blood
Tissue: Mantle Cell Lymphoma


Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Antigen Expression:CD3-, CD5+, CD10+, CD19+ (verified at ATCC )

DNA Profile (STR):Amelogenin:X
CSF1PO:9,12
D13S317:8,9
D16S539:12
D5S818:10,13
D7S820:10,11
THO1:7
TPOX:8
vWA: 14


Age:78

Gender:female

Comments:The JeKo-1 Mantle cell lymphoma (MCL) cell line was established from peripheral blood mononuclear cells of a patient with a large cell variant of MCL showing leukemic conversion. JeKo-1 cells are negative for Epstein-Barr virus and express IgM, a B-cell phenotype. The cells overexpress cyclin D1, Bcl-2, c-Myc and Rb proteins. Bcl-1/J(H) gene rearrangement was confirmed by polymerase chain reaction. JeKo-1 cells are highly tumorigenic in SCID mice. [PubMed: 9753063]

Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 20%.
Atmosphere: 5% CO2 in air recommended
Temperature: 37.0°C


Subculturing:Cultures can be maintained by the addition of fresh medium. Alternatively, cultures can be established by centrifugation with subsequent resuspension at 2 x 10(4) to 4 X 10(4) viable cells/ml. Do not allow the cell concentration to reach 2.5 X 10(6) cells/ml.

Preservation:Freeze medium: 30% RPMI; 60% FBS; 10% DMSO
Storage temperature: liquid nitrogen vapor phase


References:92895: Salaverria I, et al. Mantle cell lymphoma: from pathology and molecular pathogenesis to new therapeutic perspectives. Haematologica 91: 11-16, 2006. PubMed: 16434365
16172573: Camps, J., et al. Genomic imbalances and patterns of karyotypic variability in mantle-cell lymphoma cell lines. Leuk Res. 30(8):923-934 (2006). PubMed: 16448697
16172575: Jeon, H.J., et al. Establishment and characterization of a mantle cell lymphoma cell line. Br J Haematol. ;102(5):1323-1326 (1998 ). PubMed: 9753063



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