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MIA PaCa-2 人胰腺癌细胞 (STR)

简要描述:CRL-1420 MIA PaCa-2 人胰腺癌细胞
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  • 产品型号:CRL-1420
  • 厂商性质:生产厂家
  • 更新时间:2024-03-12
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详细介绍

细胞名称人胰腺导管癌细胞; MIA PaCa-2 (STR)
别名MIA-PaCa-2; MIA-PACA-2; MIA-Pa-Ca-2; MIA Paca2; MIA PaCa2; MiaPaCa-2; MIAPACA-2; MiaPaca.2; MiaPaCa2; Miapaca2; MIAPaCa2; MIAPACA2; Mia PACA 2; MIAPaCa-2; PaCa2; MiaPaCa
货号XF0752
规格1×10⁶cells/T25培养瓶
形态特征‍上皮细胞样
生长特性半贴壁生长
培养基DMEM+10%FBS+2.5%HS     【MIA PaCa-2培养基有售,请联系客服
冻存条件无血清细胞冻存液
培养条件气相:空气,95%;CO2,5%
温度:37℃
传代方法1:2传代;
特征特性DMEM-H: Dulbecco's Modified Eagle's Medium (DME H-21 4.5g/Liter Glucose)   10%FBS


CRL-1420  MIA PaCa-2 人胰腺癌细胞 的介绍

ATCC® Number: CRL-1420™ Price: $279.00

Designations: MIA PaCa-2

Depositors: A Yunis

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent, single cells and loosely attached clusters

Organism: Homo sapiens (human)

Morphology: attached epithelial with floating rounded cells


Source: Organ: pancreas

Disease: carcinoma

Cellular Products: human colony stimulating factor, subclass I (CSF-I); plasminogen activator

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory per

mits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Isolation: Isolation date: 1975

DNA Profile (STR): Amelogenin: X

CSF1PO: 10

D13S317: 12,13

D16S539: 10,13

D5S818: 12,13

D7S820: 12,13

THO1: 9,10

TPOX: 9

vWA: 15

Cytogenetic Analysis: This is a hypotriploid human cell line. The modal chromosome number is 61. Sixteen to 20 marker chromosomes are commonly found in a cell. A few normal chromosomes are absent.

Isoenzymes: G6PD, B

Age: 65 years

Gender: male

Ethnicity: Caucasian

Comments: The MIA PaCa-2 cell line was established by A. Yunis, et al. in 1975 from tumor tissue of the pancreas obtained from a 65-year-old Caucasian male. [58034]

The established cell line reportedly has a doubling time of about 40 hours and a colony-forming efficiency in soft agar of approximay 19%. [58034]

Hybridomas have been made against the CSF-I produced by this line ATCC HB-8207

References: 1057: Wu M, et al. Purification and characterization of a plasminogen activator secreted by cultured human pancreatic carcinoma cells. Biochemistry 16: 1908-1913, 1977. PubMed: 15590

22291: Fountzilas G, et al. Comparative effects of selected drug combinations on the growth of a human pancreatic carcinoma cell line (MIA PaCa-2). J. Natl. Cancer Inst. 76: 37-43, 1986. PubMed: 3455740

49623: Robertson JF, et al. Effect of gastrointestinal hormones and synthetic analogues on the growth of pancreatic cancer. Int. J. Cancer 63: 69-75, 1995. PubMed: 7558455


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