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您现在的位置:首页 > 产品中心 > 细胞库 > 小鼠肿瘤细胞、癌细胞 > CRL-1708QT6 鹌鹑纤维肉瘤细胞

QT6 鹌鹑纤维肉瘤细胞

  • 更新时间:  2020-09-10
  • 产品型号:  CRL-1708
  • 简单描述
  • CRL-1708 QT6 鹌鹑纤维肉瘤细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件
详细介绍

CRL-1708 QT6 鹌鹑纤维肉瘤细胞的详细介绍

CRL-1708 QT6 鹌鹑纤维肉瘤细胞

ATCC® Number:CRL-1708™   Price:$438.00
Designations:QT6
Depositors: C Moscovici
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Coturnix coturnix japonica (quail, Japanese)
Morphology:fibroblast
 
Source:Disease: fibrosarcoma
Cell Type: methylcholanthrene induced
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications:transfection host (Roche FuGENE® Transfection Reagents)
Virus Susceptibility:Rous sarcoma virus
Avian encephalomyelitis virus
Rabies virus
Marek's disease virus
Tumorigenic:Yes
Propagation:ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate supplemented with 10% tryptose phosphate broth and 5% bovine calf serum
Temperature: 37.0°C
Subculturing:Protocol: Resuspend cells by pipetting fresh medium across the monolayer. Cells may also be dislodged by hitting culture flask against the palm of the hand. Subculture at approximay 75% confluency. Use of 0.5% polyvinylpyrrolidone (PVP) in atrypsin-EDTA solution increases the viability when subculturing by enzymatic means. Neutralize and centrifuge to remove additive.Note: When subcultures are prepared using pipetting or mechanical means thecells adhere lightly to the flask substrate and exhibit a tendency toward clumping.
Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:4 is recommended
Medium Renewal: Twice per week
Preservation:Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2004
recommended serum:ATCC 30-2030
References:22385: Moscovici C, et al. Continuous tissue culture cell lines derived from chemically induced tumors of Japanese quail. Cell 11: 95-103, 1977. PubMed: 194709

CRL-1708


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