HeLa 229

HeLa 229


ATCC® Number:  CCL-2.1™      Price:  $323.00  
Designations:  HeLa 229  
Depositors:   JT Syverton  
Biosafety Level: 2 [Cells contain human papilloma virus (HPV-18) ]  
Shipped:  frozen  
Medium & Serum:  See Propagation  
Growth Properties: adherent (The line can be readily adapted to growth in suspension)
Organism: Homo sapiens (human)  
Morphology: epithelial

Source: Organ: cervix
Disease: adenocarcinoma
Cellular Products: keratin  
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  

Virus Susceptibility: Human adenovirus 7
Vesicular stomatitis virus
Human poliovirus 1
Human poliovirus 2
Human poliovirus 3
Reverse Transcript: negative  
DNA Profile (STR): Amelogenin: X
CSF1PO: 9,10
D13S317: 12,13.3
D16S539: 9,10
D5S818: 11,12
D7S820: 8,12
THO1: 7
TPOX: 8,12
vWA: 16,18
Isoenzymes:  G6PD, A
Age:  31 years  
Gender:  female  
Ethnicity:  Black  
HeLa Markers:  Y  
Comments: Relatively resistant to polioviruses.
The cells are positive for keratin by immunoperoxidase staining.
Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C