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HeLa 229

简要描述:HeLa 229

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  • 产品型号:CCL-2.1
  • 厂商性质:生产厂家
  • 更新时间:2024-08-29
  • 访  问  量:1924

详细介绍

HeLa 229

ATCC® Number:  CCL-2.1™      Price:  $323.00

Designations:

HeLa 229  

Depositors:   JT Syverton

Biosafety Level: 2 [Cells contain human papilloma virus (HPV-18) ]

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent (The line can be readily adapted to growth in suspension)

Organism: Homo sapiens (human)

Morphology: epithelial


Source: Organ: cervix

Disease: adenocarcinoma

Cellular Products: keratin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Virus Susceptibility: Human adenovirus 7

Vesicular stomatitis virus

Human poliovirus 1

Human poliovirus 2

Human poliovirus 3

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 9,10

D13S317: 12,13.3

D16S539: 9,10

D5S818: 11,12

D7S820: 8,12

THO1: 7

TPOX: 8,12

vWA: 16,18

Isoenzymes:  G6PD, A

Age:  31 years

Gender:  female

Ethnicity:  Black

HeLa Markers:  Y

Comments: Relatively resistant to polioviruses.

The cells are positive for keratin by immunoperoxidase staining.

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C





















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