4T1小鼠乳腺癌细胞

细胞名称：4T1小鼠乳腺癌细胞
介绍：4T1 是从410.4瘤株中未经诱变筛得的6-硫鸟嘌噙抗性细胞株。 当注射到BALB/c 小鼠中时，4T1自发产生高转移肿瘤，可转移到肺，肝，淋巴结和大脑，同时在注射部位形成始发灶。 诱导转移时不需要摘除始发灶。

4T1小鼠乳腺癌细胞在BALB/c小鼠中的生长与转移特性与人体中的乳腺癌十分相近。这种肿瘤是人VI期乳腺癌的动物模型。 4T1-诱导的肿瘤在手术后及未手术情况下转移的动力学相近，可以用作手术后及未手术模型。 跟其他肿瘤模型相比，由于4T1的抗6-硫鸟嘌噙特性，微小的转移细胞团(少到仅仅1个)也可以在许多远端器官中检测到。

没必要数淋巴结或称重器官
形态特性       上皮细胞
生长特性      贴壁生长
传代方法       消化3-5分钟。1:2。3天内可长满。
支原体检测      阴性

下面是ATCC的介绍

4T1 (ATCC® CRL-2539™)
Organism    Mus musculus, mouse
Tissue     mammary gland
Product Format     frozen4T1 小鼠乳腺癌细胞
Morphology     epithelial
Culture Properties     adherent
Biosafety Level     1
Disease     This tumor is an animal stage IV human breast cancer.
Strain     BALB/cfC3H
Applications    
The tumor growth and metastatic spread of 4T1 cells in BALB/c mice very closely mimic human breast cancer. This tumor is an animal model for stage IV human breast cancer.

4T1-induced tumors can be used as a post-operative model as well as a non-surgical model because the 4T1-induced tumor metastasizes spontaneously in both models with similar kinetics.
Storage Conditions     liquid nitrogen vapor temperature
Derivation    
4T1 is a 6-thioguanine resistant cell line selected from the 410.4 tumor without mutagen treatment.
Tumorigenic     Yes
Effects    4T1小鼠乳腺癌细胞
Yes, forms tumors and metastasizes in BALB/c mice
Comments    
When injected into BALB/c mice, 4T1 spontaneously produces highly metastatic tumors that can metastasize to the lung, liver, lymph nodes and brain while the primary tumor is growing in situ.
The primary tumor does not have to be removed to induce metastatic growth.
Because 4T1 is resistant to 6-thioquanine, micro-metastatic cells (as few as 1) can be detected in many distant site organs with better accuracy that most tumor models. There is no need to count nodules or weight target organs.

Complete Growth Medium     The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Phenol Red is a structural mimic for estrogen. Therefore the estrogenic activity of phenol red should be considered in any studies that utilize estrogen-responsive cells in culture (Ref). The Phenol Red-free version of RPMI 1640, is ATCC catalog No. 30-2602.

Subculturing    4T1小鼠乳腺癌细胞
NOTE: the cells should not be allowed to become confluent, subculture at 80% of confluence. Remove medium, and rinse with 0.25% trypsin-0.53mM EDTA solution. Remove the solution and add an additional 1 to 2 mL of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37°C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.
Subc*tion Ratio: A subc*tion ratio of 1:6 to 1:8 is recommended
Medium Renewal: Every 2 to 3 days
Cryopreservation    
Freeze medium: Complete growth medium 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor temperature
Culture Conditions    
Temperature: 37°C

Atmosphere: air, 95%; carbon dioxide (CO2), 5%