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Pt K1 (NBL-3) 袋鼠肾细胞

简要描述:CCL-35 Pt K1 (NBL-3) 袋鼠肾细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*培养条件
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  • 产品型号:CCL-35
  • 厂商性质:生产厂家
  • 更新时间:2021-09-10
  • 访  问  量:638

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CCL-35 Pt K1 (NBL-3) 袋鼠肾细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献i优培养条件

CCL-35 Pt K1 (NBL-3) 袋鼠肾细胞的详细介绍

CCL-35

ATCC® Number:CCL-35™  Price:$438.00
Designations:Pt K1 (NBL-3) [PTK1]

Depositors:KH Walen

Biosafety Level:1

Shipped:frozen

Medium & Serum:See Propagation

Growth Properties:adherent

Organism:Potorous tridactylis (potoroo)

Morphology:epithelial


Source:Organ: kidney
Disease: normal


Cellular Products:keratin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.



Virus Resistance:poliovirus 2

Age:adult

Gender:female

Comments:The cells are positive for keratin by immunoperoxidase staining.

Propagation:ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; newborn bovine calf serum, 10%

Subculturing:Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Rinse cell sheet twice with 0.25% trypsin, 0.03% EDTA (or Alsever's Trypsin Versene) solution, remove the trypsin solution, and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.


References:26188: . . Nature 194: 406, 1962.
26189: . . Cytogenetics 3: 19, 1964.




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