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您现在的位置:首页 > 产品中心 > 细胞库 > 动物肾细胞 > CCL-35Pt K1 (NBL-3) 袋鼠肾细胞

Pt K1 (NBL-3) 袋鼠肾细胞

  • 更新时间:  2020-09-10
  • 产品型号:  CCL-35
  • 简单描述
  • CCL-35 Pt K1 (NBL-3) 袋鼠肾细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件
    感谢您使用本单位的微生物细胞,我们有可用于科研、教学、生产的细胞5000余种,基本覆盖了环境、工业、农业、分析、食、药用真菌行业各类生产和科研用微生物,其中许多优良菌株的生产性能具有较高水平。
详细介绍

CCL-35 Pt K1 (NBL-3) 袋鼠肾细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献i优培养条件

CCL-35 Pt K1 (NBL-3) 袋鼠肾细胞的详细介绍

CCL-35

ATCC® Number:CCL-35™   Price:$438.00
Designations:Pt K1 (NBL-3) [PTK1]
Depositors: KH Walen
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Potorous tridactylis (potoroo)
Morphology:epithelial
 
Source:Organ: kidney
Disease: normal
Cellular Products:keratin      
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Virus Resistance:poliovirus 2
Age:adult
Gender:female
Comments:The cells are positive for keratin by immunoperoxidase staining.
Propagation:ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; newborn bovine calf serum, 10%
Subculturing:Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per week
Rinse cell sheet twice with 0.25% trypsin, 0.03% EDTA (or Alsever's Trypsin Versene) solution, remove the trypsin solution, and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks.
References:26188: . . Nature 194: 406, 1962.
26189: . . Cytogenetics 3: 19, 1964.  

 


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