CCL-35 Pt K1 （NBL-3） 袋鼠肾细胞，ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献i优培养条件
CCL-35 Pt K1 （NBL-3） 袋鼠肾细胞的详细介绍
CCL-35
| ATCC® Number: | CCL-35™ | Price: | $438.00 |
| Designations: | Pt K1 （NBL-3） [PTK1] |
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| | Depositors: | KH Walen |
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| | Biosafety Level: | 1 |
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| | Shipped: | frozen |
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| | Medium & Serum: | See Propagation |
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| | Growth Properties: | adherent |
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| | Organism: | Potorous tridactylis （potoroo） |
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| | Morphology: | epithelial
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| | Source: | Organ: kidney
Disease: normal |
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| | Cellular Products: | keratin |
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| | Permits/Forms: | In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location. |
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| | Virus Resistance: | poliovirus 2 |
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| | Age: | adult |
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| | Gender: | female |
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| | Comments: | The cells are positive for keratin by immunoperoxidase staining. |
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| | Propagation: | ATCC complete growth medium: Minimum essential medium （Eagle） in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; newborn bovine calf serum, 10% |
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| | Subculturing: | Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended
Medium Renewal: Twice per weekRinse cell sheet twice with 0.25% trypsin, 0.03% EDTA （or Alsever's Trypsin Versene） solution, remove the trypsin solution, and allow the culture to stand for 5 to 10 minutes at room temperature. Add fresh medium, aspirate and dispense into new flasks. |
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| | References: | 26188: . . Nature 194: 406, 1962. 26189: . . Cytogenetics 3: 19, 1964. |
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