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RA3 3A1/6.1 大鼠X小鼠杂合脾,骨髓瘤细胞

简要描述:TIB-146 RA3 3A1/6.1 大鼠X小鼠杂合脾,骨髓瘤细胞, ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和*培养条件

  • 产品型号:TIB-146
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  • 更新时间:2021-07-11
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TIB-146 RA3 3A1/6.1 大鼠X小鼠杂合脾,骨髓瘤细胞, ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和优培养条件

TIB-146 RA3 3A1/6.1 大鼠X小鼠杂合脾,骨髓瘤细胞 的详细介绍

TIB-146

ATCC® Num
Depositors: RL Coffman
ber:
TIB-146™   Price:$399.00
Designations:RA3-3A1/6.1
Isotype:IgM
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:suspension
Organism:Rattus norvegicus (B cell); Mus musculus (myeloma) (rat (B cell); mouse (myeloma))
Morphology:lymphoblast
 
Source:Cell Type: hybridoma: B lymphocyte;
Cellular Products:immunoglobulin; monoclonal antibody; against the B220 mouse B cell antigen (200000 dalton form of the T200 glycoprotein, CD45R)
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Comments:Animals were immunized with the mouse cell line RAW 112.
Spleen cells were fused with the S194/5.XX0.BU.1 myeloma.
Tested and found negative for ectromelia virus (mousepox).
Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Dulbecco's Modified Eagle's Medium, Catalog No. 30-2002. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Subculturing:Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 3 x 10 exp4 cells/ml, and do not allow the cell concentration to exceed 5 x 10 exp5 cells/ml.
References:925: Coffman RL, Weissman IL. A monoclonal antibody that recognizes B cells and B cell precursors in mice. J. Exp. Med. 153: 269-279, 1981. PubMed: 6787164
927: Coffman RL, Weissman IL. B220: a B cell-specific member of the T200 glycoprotein family. Nature 289: 681-683, 1981. PubMed: 6970340
32948: Wong P, Rudensky AY. Phenotype and function of CD4+ T cells in mice lacking invariant chain. J. Immunol. 156: 2133-2142, 1996. PubMed: 8690902

 

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