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您现在的位置:首页 > 产品中心 > 细胞库 > 大鼠肿瘤细胞 > CRL-2755EMT6 大鼠乳腺癌细胞

EMT6 大鼠乳腺癌细胞

  • 更新时间:  2020-10-16
  • 产品型号:  CRL-2755
  • 简单描述
  • CRL-2755 EMT6 大鼠乳腺癌细胞, ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件
详细介绍

CRL-2755 EMT6 大鼠乳腺癌细胞, ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献优培养条件

CRL-2755 EMT6 大鼠乳腺癌细胞 的详细介绍

CRL-2755

ATCC® Number:CRL-2755™   Price:$355.00
Designations:EMT6
Depositors: S Rockwell
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Mus musculus (mouse)
Morphology:epithelial
 
Source:Organ: breast
Strain: BALB/cCrgl
Disease: mammary carcinoma
Cell Type: epithelial
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Isolation:Isolation date: 1971
Tumorigenic:Yes
Gender:female
Comments: 
Subculturing:Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:4 to 1:10 is recommended
Medium Renewal: Every 2 to 3 days
Preservation:Freeze medium: Complete growth medium supplemented with 5% (v/v) DMSO
Storage temperature: liquid nitrogen vapor phase
Related Products:recommended serum:ATCC 30-2020
References: 

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