咨询热线

15000266580

当前位置:首页 >产品中心>细胞库>大鼠正常细胞>HIT-T15 大鼠胰腺B细胞

HIT-T15 大鼠胰腺B细胞

简要描述:HIT-T15 大鼠胰腺B细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件

  • 产品型号:
  • 厂商性质:生产厂家
  • 更新时间:2021-09-10
  • 访  问  量:528

产品分类

Product Category

相关文章

Related Articles

详细介绍

HIT-T15 大鼠胰腺B细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献i优培养条件


HIT-T15 大鼠胰腺B细胞

ATCC® Number: CRL-1777™    Price: $289.00
Designations: HIT-T15

Depositors: R Santerre

Biosafety Level:2 [Cells Contain PAPOVAVIRUS ]

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties:adherent

Organism:Mesocricetus auratus (hamster, Syrian golden)

Morphology:epithelial
HIT-T15   大鼠胰腺B细胞


Source:Organ: pancreas
Tissue: islet of Langerhans
Cell Type: beta cell;


Cellular Products:insulin

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications:transfection host (Roche FuGENE® Transfection Reagents)

Receptors:glucagon; somatostatin; glucocorticoid

Comments:This line was established from a primary culture of Syrian hamster islet cells which were transformed with SV40.
The cells secrete up to 24 ng of insulin per 10 exp6 cells per 24 hours (passage 60).
Insulin secretion is stimulated by glucose and glucagon, and is suppressed by somatostatin and glucocorticoids.
Insulin synthesis decreases with length of time in culture.


Propagation: ATCC complete growth medium: Ham's F12K medium with 2 mM L-glutamine adjusted to contain 1.5 g/L sodium bicarbonate, 87.5%; dialyzed horse serum, 10%; fetal bovine serum, 2.5%
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C


Subculturing: Protocol:
  1. Remove and discard culture medium.

  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

  5. Add appropriate aliquots of the cell suspension to new culture vessels.

  6. Incubate cultures at 37°C.






产品咨询

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
联系方式

邮箱:xiangfbio@163.com

地址:上海市虹口区赤峰路630弄南雁大厦

咨询热线

400-821-8510

(周一至周日9:00- 19:00)

在线咨询
  • 扫一扫 微信咨询

Copyright©2021 上海复祥生物科技有限公司 All Right Reserved    备案号:沪ICP备10013034号-2     sitemap.xml
技术支持:化工仪器网    管理登陆