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PK136 小鼠X小鼠细胞

简要描述:HB-191 PK136 小鼠X小鼠细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件

  • 产品型号:HB-191
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  • 更新时间:2021-09-12
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HB-191 PK136 小鼠X小鼠细胞,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献优培养条件

HB-191 PK136 小鼠X小鼠细胞

ATCC® Number: HB-191™    Price: $323.00
Designations: PK136

Depositors: GC Koo

Isotype: IgG2a

Biosafety Level:1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties:suspension

Organism:Mus musculus (B cell); Mus musculus (myeloma) (mouse (B cell); mouse (myeloma))

Morphology:lymphoblast


Source:Cell Type: hybridoma: B lymphocyte;

Cellular Products:immunoglobulin; monoclonal antibody; against mouse natural killer (NK) cells

Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
HB-191

Comments:Animals were immunized with spleen and bone marrow cells from CE mice.
Spleen cells were fused with Sp2/0-Ag14 myeloma cells.
The antibody is specific for NK cells and is cytotoxic in the presence of complement.
The pattern of reactivity of the antibody with cells of various strains of mice matches the pattern of conventional NK 1.1 antisera.
Tested and found negative for ectromelia virus (mousepox).


Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC Hybri-Care Medium, Catalog No. 46-X. Hybri-Care Medium is supplied as a powder and should be reconstituted in 1 L cell-culture-grade water. To make the complete growth medium, add the following components to the base medium:
  • fetal bovine serum to a final concentration of 10%

  • 1.5 g/L sodium bicarbonate for use with 5% CO2 in air atmosphere.
    Temperature: 37.0°C



Subculturing: Protocol: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 1 X 10(5) cells/ml and maintain between 5 X 10(4) and 1 X 10(6) cells/ml. Do not exceed 1 X 10(6) cells/ml.
Medium Renewal: Every 2 to 3 days


Related Products:recommended serum:ATCC 30-2020
Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 46-X


References:1319: Koo GC, et al. The NK-1.1 (-) mouse: a model to study differentiation of murine NK cells. J. Immunol. 137: 3742-3747, 1986. PubMed: 3782794
58145: Koo GC, Peppard JR. Establishment of monoclonal anti-NK-1.1 antibody. Hybridoma 3: 301-303, 1984. PubMed: 6500587





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