您好,欢迎进入上海复祥生物科技有限公司网站!
全国服务热线:15000266580
上海复祥生物科技有限公司
产品搜索
PRODUCT SEARCH
产品分类
PRODUCT CLASSIFICATION
查看更多 >>
相关文章
RELEVANT ARTICLES
您现在的位置:首页 > 产品中心 > 细胞库 > 人肿瘤细胞、癌细胞 > BC-1人lymphoma B 淋巴细胞

人lymphoma B 淋巴细胞

  • 更新时间:  2020-09-04
  • 产品型号:  BC-1
  • 简单描述
  • CRL-2230 BC-1 人lymphoma B 淋巴细胞,
    ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,
    细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件!
详细介绍

CRL-2230 BC-1 人lymphoma B 淋巴细胞

ATCrC® Numbe: CRL-2230™ Price: $417.00

Designations: BC-1
Depositoo Level: 2 [CELLS CONTAIN HERPESVIRUS ]

Shipped: frzen
Medium & Serum: See Propagation
Growth Properties: suspension
Organism: Homo sapiens (human)
Morphology: lymphoblast

CRL-2230 BC-1 人lymphomars: E Cesarman
Biosafety B 淋巴细胞

Source: Disease: lymphoma
Cell Type: B lymphocyte;
Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (Roche FuGENE® Transfection Reagents)
Antigen Expression: CD30 +; CD45 +; EMA + (epithelial membrane antigen)
DNA Profile (STR): Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 8
D16S539: 12,13
D5S818: 11,12
D7S820: 11
THO1: 9.3
TPOX: 8,9
vWA: 16,20
Age: 46 years
Gender: male
Comments: BC-1 is a lymphoma cell line originated in 1992 by Ethel Cesarman and Giorgio Inghirami in the laboratory of Daniel M. Knowles.
The cells were derived from an acquired immunodeficiency syndrome (AIDS) related body cavity based lymphoma presenting as a primary lymphomatous effusion in the peritoneal cavity.
The cells contain two viral genomes: Epstein-Barr virus (EBV) and Kaposi sarcoma associated herpesvirus (KSHV, provisionally designed HHV-8).
KSHV is a recently identified and largely uncharacterized virus.
The BC-1 cell line allows in vitro culture of KSHV viral genomes.
It is also a practical source of KSHV DNA to be used as a positive control for screening tests.
Propagation: ATCC complete growth medium: RPMI 1640 medium, 80%; fetal bovine serum, 20%

Subculturing: Medium Renewal: Every 2 to 3 days
Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 2 X 10 exp6 cells/ml.
Preservation: Culture medium, 95%; DMSO, 5%
Doubling Time: 48 to 72 hrs
References: 22572: Moore PS, et al. Primary characterization of a herpesvirus agent associated with Kaposi's sarcoma. J. Virol. 70: 549-558, 1996. PubMed: 8523568
23358: Cesarman E, et al. In vitro establishment and characterization of two acquired immunodeficiency syndrome-related lymphoma cell lines (BC-1 and BC-2) containing Kaposi's sarcoma-associated herpesvirus-like (KSHV) DNA sequences. Blood 86: 2708-2714, 1995. PubMed: 7670109
23395: Chadburn A, et al. CD30 (Ki-1) positive anaplastic large cell lymphomas in individuals infected with the human immunodeficiency virus. Cancer 72: 3078-3090, 1993. PubMed: 8221575
23493: Cesarman E, et al. Kaposi's sarcoma-associated herpesvirus-like DNA sequences in AIDS- related body-cavity-based lymphomas. N. Engl. J. Med. 332: 1186-1191, 1995. PubMed: 7700311

CRL-2230 BC-1 人lymphoma B 淋巴细胞


留言框

  • 产品:

  • 留言内容:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 详细地址:

  • 省份:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
Contact Us
  • 联系QQ:316826860/1784382136/1784382138
  • 联系邮箱:xiangfbio@163.com
  • 传真:021-35080520
  • 联系地址:上海市虹口区赤峰路630弄南雁大厦

扫一扫  微信咨询

©2020 上海复祥生物科技有限公司 版权所有  备案号:沪ICP备10013034号-2   技术支持:化工仪器网    GoogleSitemap    总访问量:178570 管理登陆