咨询热线

15000266580

当前位置:首页 >产品中心>细胞库>人正常细胞>MC/CAR人外周血淋巴细胞

人外周血淋巴细胞

简要描述:CRL-8083 MC/CAR 人外周血淋巴细胞,
ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|;
细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件!

  • 产品型号:MC/CAR
  • 厂商性质:生产厂家
  • 更新时间:2024-03-11
  • 访  问  量:1396

产品分类

Product Category

相关文章

Related Articles

详细介绍

CRL-8083 MC/CAR 人外周血淋巴细胞

ATCC® Number: CRL-8083™ Price: $417.00

Designations: MC/CAR

Depositors: Research Corporation

Isotype: IgG1 (cytoplasmic); kappa light chain

Biosafety Leee Propagation

Growth Properties: suspension

Organism: Homo sapiens (human)

Morphology: lymphoblast

CRL-8083 MC/CAR 人外周血淋巴细胞vel: 2

Shipped: frozen

Medium & Serum: S

Source: Organ: peripheral blood

Disease: plasmacytoma; myeloma

Cell Type: B lymphocyte;

Cellular Products: immunoglobulin

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Receptors: complement (C3b)

Tumorigenic: Yes

Antigen Expression: HLA A1, A33/31, B7, B18, DRw1, DRw2; CD11a +; CD19 +; CD20 + CD28 -; CD38 +

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 11,12

D13S317: 11,13

D16S539: 11,12

D5S818: 11,14

D7S820: 9,13

THO1: 6,9

TPOX: 8

vWA: 17,18

Cytogenetic Analysis: normal male diploid

Isoenzymes: ADA, 1

AK-1, 1

ES-D, 1

G6PD, B

GLO-I, 1

PGM1, 1

PGM3, 1

Age: 81 years

Gender: male

Ethnicity: Caucasian

Comments: Although derived from cells taken from a patient with a plasmacytoma, this line has been shown to be an EBV-transformed B lymphoblastoid cell line. [43270]

The cells produce, but do not secrete, immunoglobulin.

No surface immunoglobulin.

8-azaguanine mutants may be cloned out or mutagenized for HAT sensitive cultures.

Can be used as a human fusion line.

Propagation: ATCC complete growth medium: Iscove's modified Dulbecco's medium, 80%; fetal bovine serum, 20%

Temperature: 37.0°C

Subculturing: Cultures can be maintained by addition or replacement of fresh medium. Start cultures at 5 X 10 exp5 cells/ml and maintain between 5 X 10 exp5 and 1 X 10 exp6 cells/ml.

References: 3535: Ritts RE Jr.. Human nonsecretory plasmacytoid cell line. US Patent 4,434,230 dated Feb 28 1984

43268: Pellat-Deceunynk C, et al. Human myeloma cell lines as a tool for studying the biology of multiple myeloma: a reappraisal 18 years after. Blood 86: 4001-4002, 1995. PubMed: 7579375

43270: Drexler H, et al. False human hematopoietic cell lines: cross-contaminations and misinterpretations. Leukemia 13: 1601-1607, 1999. PubMed: 10516762

58062: Ritts RE J, et al. Establishment and characterization of a human non-secretory plasmacytoid cell line and its hybridization with human B cells. Int. J. Cancer 31: 133-141, 1983. PubMed: 6600719



CRL-8083 MC/CAR
















产品咨询

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
联系方式

邮箱:xiangfbio@163.com

地址:上海市虹口区四平路710号7层

咨询热线

400-821-8510

(周一至周日9:00- 19:00)

在线咨询
  • 扫一扫 微信咨询

Copyright©2024 上海复祥生物科技有限公司 All Right Reserved    备案号:沪ICP备10013034号-2     sitemap.xml
技术支持:化工仪器网    管理登陆