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您现在的位置:首页 > 产品中心 > 细胞库 > 人肿瘤细胞、癌细胞 > CRL-1676WM-266-4细胞, 人黑素瘤细胞

WM-266-4细胞, 人黑素瘤细胞

  • 更新时间:  2020-08-04
  • 产品型号:  CRL-1676
  • 简单描述
  • WM-266-4细胞, 人黑素瘤细胞
    原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件!
详细介绍

WM-266-4细胞, 人黑素瘤细胞

ATCC® Number:CRL-1676™    Price:$318.00
Designations:WM-266-4
Depositors: M Herlyn
Biosafety Level:1
Shipped:frozen
Medium & Serum:See Propagation
Growth aProperties:adherent
Organism:Homo sapiens (humn)
Morphology:epithelial
 
Source:Organ: skin 
Disease: melanoma
Cellular Products:proteoglycan antigen characteristic of melanoma
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
         WM-266-4细胞, 人黑素瘤细胞
Tumorigenic:Yes
DNA Profile (STR):Amelogenin: X 
CSF1PO: 12 
D13S317: 12,13 
D16S539: 11,12 
D5S818: 13 
D7S820: 8 
THO1: 7,9 
TPOX: 8,11 
vWA: 15,17
Cytogenetic Analysis:The cell line shares many properties with the parent tumor line including same karyology. This is a hypertriploid human cell line. The modal chromosome number is 82, occurring at 32%, with polyploidy at 8.6%. (Cells with 80 chromosome counts also occurred at a high frequency.) There were two paired [der(1)t(1;?) (p32;?), and der(11)t(2;11) (q22.1;p13)], and three to four single markers consistently found in each cell. Several other markers were also found in some cells only, including a small metacentric chromosome of unknown origin. Neither DMs nor HSRs were detected. Among the normal chromosomes, N11 had only single copy, and N1, N6, N8, N9, and N16 had two copies each. As expected, other normal chromosomes had three to four copies per cell. Two X chromosomes were generally found in each cell.
Age:58 years
Gender:female
Comments:The WM-266-4 cell line was derived from a metastatic site of a malignant melanoma. 
Another cell line derived from the primary tumor of the same patient also is available (ATCC CRL-1675).
Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Temperature: 37.0°C
Subculturing:Protocol:                                          
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37°C.

Subcultivation Ratio: A subcultivation ratio of 1:3 to 1:6 is recommended 
Medium Renewal: 2 to 3 times per week
Preservation:Freeze medium: Complete growth medium, 95%; DMSO, 5% 
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
derived from same individual:ATCC CRL-1675

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