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WISH 人羊膜细胞

简要描述:CCL-25 WISH 人羊膜细胞,原代细胞|细胞系|细胞株|菌种;细胞库管理规范,提供的细胞株背景清楚,提供参考文献和培养条件!

  • 产品型号:CCL-25
  • 厂商性质:生产厂家
  • 更新时间:2022-03-07
  • 访  问  量:292

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CCL-25 WISH 人羊膜细胞

ATCC® Number:CCL-25™    Price:$323.00
Additional information about this cell lineDesignations:WISHDepositors: L HayflickBiosafety Level:2Shipped:frozenMedium & Serum:See PropagationGrowth Properties:adherentOrganism:Homo sapiens (human)Morphology:epithelial Source:Organ: HeLa contaminantCellular Products:keratinPermits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.CCL-25 WISH 人羊膜细胞Virus Susceptibility:poliovirus 1, 2; adenovirus 3; vesicular stomatitis (Indiana)Reverse Transcript:negativeDNA Profile (STR):Amelogenin: X CSF1PO: 9,10 D13S317: 13.3 D16S539: 9,10 D5S818: 11,12 D7S820: 8,12 THO1: 7 TPOX: 8,12 vWA: 16,18Isoenzymes:G6PD, AHeLa Markers:YComments:This line was originally thought to be derived from normal amnion, but was subsequently found, based on isoenzyme analysis, HeLa marker chromosomes, and DNA fingerprinting, to have been established via HeLa cell contamination. The cells are positive for keratin by immunoperoxidase staining. NOTE: Cells of this line contain HeLa marker chromosomes, and were derived via HeLa contaminationPropagation:ATCC complete growth medium: Minimum essential medium (Eagle) in Earle's BSS with non-essential amino acids and 1 mM sodium pyruvate, 90%; fetal bovine serum, 10%Temperature: 37.0°CSubculturing:Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:10 is recommended Medium Renewal: Twice per week Remove medium, add fresh 0.25% trypsin solution for 3 to 5 minutes, remove trypsin and let culture sit at room temperature for 5 to 10 minutes. Add fresh medium, aspirate and dispense into new flasks.Preservation:culture medium 95%; DMSO, 5%Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003recommended serum:ATCC 30-2020References:22153: Hayflick L. The establishment of a line (WISH) of human amnion cells in continuous cultivation. Exp. Cell Res. 23: 14-20, 1961. PubMed:1371249026034: . . Am. J. Dis. Child. 103: 460, 1962.


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