IMR-90人胚肺成纤维细胞

IMR-90细胞（IMR-90人胚肺成纤维细胞）ATCC® CCL-186™ 详细资料

基本信息​

• ATCC编号：CCL-186™
• ​ designation：IMR-90
• ​ depositors：WW Nichols（W·W·尼科尔斯）
• 生物安全等级：1级（BSL-1）
• 运输方式：冷冻（干冰）
• 生长特性：贴壁生长（adherent）
• 种属：人（Homo sapiens）
• 形态：成纤维细胞样（fibroblast）
• 组织来源：肺（lung）
• 疾病状态：正常（normal）
• 细胞类型：成纤维细胞（fibroblast）

许可与法规​

应用​

• 转染宿主（适用于Lonza公司的Nucleofection技术）。

病毒易感性​

• 脊髓灰质炎病毒1型（Human poliovirus 1）
• 脊髓灰质炎病毒2型（Human poliovirus 2）
• 水痘-带状疱疹病毒（Varicella-Zoster）
• 单纯疱疹病毒1型（Herpes simplex virus 1）
• 单纯疱疹病毒2型（Herpes simplex virus 2）
• 脊髓灰质炎病毒3型（Human poliovirus 3）
• 痘苗病毒（Vaccinia virus）
• 巨细胞病毒（Human herpesvirus 5，CMV）
• 水泡性口炎病毒（Vesicular stomatitis virus）

分子特征​

• 反转录酶活性：阴性（Reverse Transcript: negative）
• STR DNA谱型（短串联重复序列）：
  • Amelogenin（性别位点）：X
  • CSF1PO：11,13
  • D13S317：11,13
  • D16S539：10,13
  • D5S818：12,13
  • D7S820：9,12
  • THO1：8,9.3
  • TPOX：8,9
  • vWA：16,19
• 细胞遗传学分析：正常女性二倍体（46,XX），染色体稳定（normal human female; diploid; stable）
• 同工酶：葡萄糖-6-磷酸脱氢酶（G6PD）类型为B（Isoenzymes: G6PD, B）

来源与背景​

培养条件​

1. 完全生长培养基​

2. 培养环境​

• 气体氛围：空气（95%）+ 二氧化碳（CO₂，5%）
• 温度：37.0°C

3. 传代（Subculturing）​

1. 移除并丢弃旧培养液。
2. 用0.25%（w/v）胰蛋白酶-0.53 mM EDTA溶液短暂冲洗细胞层，以去除含胰酶抑制剂的血清残留。
3. 向培养瓶中加入2.0-3.0 ml胰蛋白酶-EDTA溶液，在倒置显微镜下观察，直至细胞层分散（通常需5-15分钟）。
   注意：避免摇晃或敲击培养瓶，防止细胞结团。难以分散的细胞可置于37°C培养箱中加速解离。
4. 加入6.0-8.0 ml完全生长培养基，轻轻吹打细胞以终止胰酶消化。
5. 将细胞悬液分装至新的培养容器（如培养瓶或培养皿）。
6. 置于37°C、5% CO₂培养箱中培养。

4. 冻存（Preservation）​

• 冻存液：完全生长培养基（95%）+ 二甲基亚砜（DMSO，5%）。
• 储存温度：液氮蒸气相（liquid nitrogen vapor phase）。

相关产品​

• 推荐基础培养基（不含额外补充剂或血清）：ATCC 30-2003（Eagle's Minimum Essential Medium）。
• 推荐血清：ATCC 30-2020（胎牛血清）。

参考文献​

1. Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science196: 60-63, 1977.（PubMed: 841339）
2. Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol Cell Biol16: 4832-4841, 1996.（PubMed: 8756642）
3. Ostlund RE Jr., et al. A stereospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J Biol Chem271: 10073-10078, 1996.（PubMed: 8626564）

其他信息​

• 细胞库信息：中国科学院典型培养物保藏委员会细胞库（GNHu32）提供该细胞，经支原体、细菌、真菌检测均为阴性，且通过STR鉴定无误。
• 供应限制：仅供研究使用（For research use only）。

IMR-90细胞， 人胚肺成纤维细胞

ATCC® Number:  CCL-186™

Designations:  IMR-90

Depositors:   WW Nichols

Biosafety Level: 1

Shipped:  frozen

Medium & Serum:  See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

Source: Organ: lung

Disease: normal

Cell Type: fibroblast

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.

Applications: transfection host (Nucleofection technology from Lonza)

Virus Susceptibility: Human poliovirus 1

Human poliovirus 2

Varicella-Zoster

Herpes simplex virus 1

Herpes simplex virus 2

Human poliovirus 3

Vaccinia virus

Human herpesvirus 5

Vesicular stomatitis virus

Reverse Transcript: negative

DNA Profile (STR): Amelogenin: X

CSF1PO: 11,13

D13S317: 11,13

D16S539: 10,13

D5S818: 12,13

D7S820: 9,12

THO1: 8,9.3

TPOX: 8,9

vWA: 16,19

Cytogenetic Analysis: normal human female; diploid; stable

Isoenzymes:  G6PD, B

Age:  16 weeks gestation

Gender:  female

Ethnicity:  Caucasian

Comments: The human diploid fibroblast strain IMR-90 was derived by W.W. Nichols and associates from the lungs of a 16-week female fetus. [22381]

The division potential, viral susceptibilities and other properties have been thoroughly studied such that the line may be considered as an alternate for WI-38 and other standard human lung cell strains.

The cells have been reported to be capable of attaining 58 population doublings before the onset of senescence. [22381]

Propagation:  ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Atmosphere: air, 95%; carbon dioxide (CO2), 5%

Temperature: 37.0°C

Subculturing:  Protocol:

Remove and discard culture medium.

Briefly rinse the cell layer with 0.25% (w/v) Trypsin- 0.53 mM EDTA solution to remove all traces of serum that contains trypsin inhibitor.

Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).

Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37°C to facilitate dispersal.

Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.

Add appropriate aliquots of the cell suspension to new culture vessels.

Incubate cultures at 37°C.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:8 is recommended

Medium Renewal: Every 3 to 4 days

Preservation:  Freeze medium: Complete growth medium 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor phase

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003

recommended serum:ATCC 30-2020

References: 22381: Nichols WW, et al. Characterization of a new human diploid cell strain, IMR-90. Science 196: 60-63, 1977. PubMed: 841339

32932: Dolganov GM, et al. Human Rad50 is physically associated with human Mre11: identification of a conserved multiprotein complex implicated in recombinational DNA repair. Mol. Cell. Biol. 16: 4832-4841, 1996. PubMed: 8756642

33041: Ostlund RE Jr., et al. A sterospecific myo-inositol/D-chiro-inositol transporter in HepG2 liver cells. J. Biol. Chem. 271: 10073-10078, 1996. PubMed: 8626564

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