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您现在的位置:首页 > 产品中心 > 细胞库 > 兔肾细胞系 > CCL-37 RK-13 兔肾细胞系

CCL-37 RK-13 兔肾细胞系

  • 更新时间:  2020-12-07
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  • 简单描述
  • CCL-37 RK-13 兔肾细胞系,ATCC 细胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背景清楚,提供参考文献和Z优培养条件
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CCL-37 RK-13 兔肾胞|细胞系|细胞株|肿瘤细胞|细胞|贴壁细胞|悬浮细胞|,细胞库管理规范,提供的细胞株背细胞系,ATCC 细景清楚,提供参考文献和优培养条件

 

ATCC® Number:CCL-37™   Price:$339.00
Designations:RK13
Depositors: BC Meyer
Biosafety Level:2 [Cells contain Bovine Viral Diarrhea Virus (BVDV) ]
Shipped:frozen
Medium & Serum:See Propagation
Growth Properties:adherent
Organism:Oryctolagus cuniculus (rabbit)
Morphology:epithelial
 
Source:Organ: kidney
Disease: normal
Cellular Products:keratin
Permits/Forms:In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.
 
Applications:transfection host (Roche FuGENE® Transfection Reagents)
Age:5 weeks        CCL-37 RK-13 兔肾细胞系
Comments:The cells are positive for keratin by immunoperoxidase staining.The cells are positive for bovine viral diarrhea virus (BVDV).
Propagation:ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
Atmosphere: air, 95%; carbon dioxide (CO2), 5%
Temperature: 37.0°C
Subculturing:Protocol:
  1. Remove and discard culture medium.
  2. Briefly rinse the cell layer with 0.25% (w/v) Trypsin - 0.53 mM EDTA solution to remove all traces of serum which contains trypsin inhibitor.
  3. Add 2.0 to 3.0 ml of Trypsin-EDTA solution to flask and observe cells under an inverted microscope until cell layer is dispersed (usually within 5 to 15 minutes).
    Note: To avoid clumping do not agitate the cells by hitting or shaking the flask while waiting for the cells to detach. Cells that are difficult to detach may be placed at 37C to facilitate dispersal.
  4. Add 6.0 to 8.0 ml of complete growth medium and aspirate cells by gently pipetting.
  5. Add appropriate aliquots of the cell suspension to new culture vessels.
  6. Incubate cultures at 37C.

Subcultivation Ratio: A subcultivation ratio of 1:2 to 1:8 is recommended
Medium Renewal: Twice per week
Preservation:Freeze medium: culture medium, 95%; DMSO, 5%
Storage temperature: liquid nitrogen vapor phase
Related Products:Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2003
recommended serum:ATCC 30-2020
0.25% (w/v) Trypsin - 0.53 mM EDTA in Hank' BSS (w/o Ca++, Mg++):ATCC 30-2101
Cell culture tested DMSO:ATCC 4-X
References:18385: Didier ES, et al. Characterization of Encephalitozoon (Septata) intestinailis isolates cultured from nasal mucosa and bronchoalveolar lavage fluids of two AIDS patients. J. Eukaryot. Microbiol. 43: 34-43, 1996. PubMed: 8563708
22145: . . Lancet 2: 640, 1963. 
22883: Bolin SR, et al. Survey of cell lines in the American Type Culture Collection for bovine viral diarrhea virus. J. Virol. Methods 48: 211-221, 1994. PubMed: 7989438
26192: . . Lancet 2: 593, 1963.
28301: Loffler S, et al. CD9, a tetraspan transmembrane protein, renders cells susceptible to canine distemper virus. J. Virol. 71: 42-49, 1997. PubMed: 8985321        

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