ATCC 6538 金黄色葡萄球菌

ATCC 6538 金黄色葡萄球菌使用说明

组成：冻干菌种瓶、脑心浸液（用于复溶菌种及保存用）、使用说明书。

方法：建议用下列方法恢复培养冷冻干燥的菌种。

复苏菌种前应准备适于该菌种生长的固体或液体培养基和相应的培养设备，所有操作均应在符合生物安全保护及无菌条件下进行。

－、菌种名称：ATCC 6538 金黄色葡萄球菌
                

二、菌株恢复培养

　　1．拉开易撕铝盖，用浸过75％酒精的脱脂棉擦净丁基胶塞及疫苗瓶。吸取500微升的菌种复溶液，滴人疫苗瓶内，轻轻振荡，使冻于菌体溶解呈悬浮状。

　　2．取适量菌体悬浮液(建议岢养菌种可适当加大用量)，移植于适宜的营养琼脂斜面上，放置在35-37℃培养24-48小时 （注意：首次复苏培养不能用液体培养基培养）

3．斜面（一代）放于4℃每月转接一次以此类推，建议使用菌种代数不超过5代，5代后菌种应销毁。

三、注意事项

1． 菌种活化前，请保存在2一8℃的环境下。

2． 嗜血杆菌、奈瑟氏菌属等初代培养需要5-10%CO₂环境和专用的培养基，建议使用本公司的专用培养基。

3． 厌氧菌的培养，如无特别说明，自开封至接种完成，均需以无氧气体充填，以保持厌氧状态。

4． 某些菌种经过冷冻干燥保存后，延迟期较长，需连续两次继代培养才能正常生长后使用。

ATCC 6538 金黄色葡萄球菌

ATCC® Number:   6538™      Price:  $155.00  
Organism:  Staphylococcus aureus subsp. aureus Rosenbach 
Designations:  FDA 209  
Isolation:  human lesion  
Depositor:  FDA  
History:  ATCC <<--FDA<<--AMC  
Biosafety Level:  2  
Shipped:  freeze-dried  
Growth Conditions:  ATCC medium18: Trypticase soy agar
Temperature: 37.0°C  
Permits/Forms:  In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.  
Cross References:  Nucleotide （GenBank） : DQ212950Staphylococcus aureus strain ATCC 6538 16S ribosomal RNA gene, partial sequence.  
Comments:  This strain is recommended by ATCC for use in the tests described in military specification MIL G-13734B where only the taxon is specified.  
Applications:  Filtration Efficiency Testing [92561] 
detection of [92783] 
efficacy testing [92583] [92584] [92587] [92779] 
inhibition testing [92580] [92839] 
media testing [11018] [11019] [21509] [21511] [21613] [92390] [92845] 
preparatory test control [21613] 
quality control strain  

ATCC 6538 金黄色葡萄球菌
testing [92304] [92305] [92307] [92402] [92403] 
testing antimicrobial agents [92592] [92595] 
testing antimicrobial handwashing formulations [92590] 
testing disinfectants [6664] [6669] [21475] [21478] [21496] [21579] [21580] [21581] [21582] [21584] [23722] [92280] [92356] [92357] [92362] [92588] [92593] 
testing sanitizers [4085] [92358] [92360] [92361] [92363] [92588] [92593] 
testing bactericides [92444] [92445] [92733] [92784] [92789] [92830] [92835] [92837] 
quality control strain for Biosynth products  
Related Products:  also available as SafeTsource™:ATCC 6538NA
purified DNA: ATCC 6538D-5  
References:  1968: Connolly P, et al. The use of impedance for preservative efficacy testing of pharmaceuticals and cosmetic products. J. Appl. Bacteriol. 76: 68-74, 1994. PubMed: 8144407