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人成巨核细胞白血病细胞

简要描述:CRL-2021 MEG-01 人成巨核细胞白血病细胞,
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  • 产品型号:MEG-01
  • 厂商性质:生产厂家
  • 更新时间:2024-03-11
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CRL-2021 MEG-01 人成巨核细胞白血病细胞

ATCC® Number: CRL-2021™

Designations: MEG-01

Depositors: M Tekeuchi, MJ Fitzgerald

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: mixed, adherent and suspension

Organism: Homo sapiens (human)

Morphology: lymphoblast

CRL-2021 MEG-01 人成巨核细胞白血病细胞

Source: Disease: chronic myelogenous leukemia (CML)

Cell Type: megakaryoblast;

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


CRL-2021 MEG-01

Applications: transfection host (Nucleofection technology from Lonza)

Antigen Expression: CD41 +; CD61 +; CDw14 +

DNA Profile (STR): Amelogenin: X,Y

CSF1PO: 10

D13S317: 8

D16S539: 9

D5S818: 13

D7S820: 11

THO1: 7

TPOX: 8,11

vWA: 16

Cytogenetic Analysis: hyperdiploid; modal number = 56 to 58; the Philadelphia chromosome (Ph1) is present

Age: 55 years

Gender: male

Comments: The MEG-01 cell line was derived in 1983 at the Nagoya University School of Medicine, Nagoya, Japan from bone marrow cells taken from a patient in megakaryoblastic crisis of CML.

The cells are positive for cytoplasmic Factor VIII and surface GPIIb/IIIa, periodic acid - Schiff (PAS) reaction, alpha naphthyl acetate esterase and acid phosphatase.

They are negative for myeloperoxidase, alpha naphthyl butyrate esterase, naphthol AS-D chloroacetate esterase and alkaline phosphatase.

The stain positively with monoclonal antibodies BA-1 (anti B cell, granulocyte), HPL-3 (anti gpIIb/IIIa) and 20.3 (anti monocyte, plaet).

They are negative for other lymphoid and myeloid seris antigens.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated RPMI-1640 Medium, Catalog No. 30-2001. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Subcultures are prepared by scraping the adherent cells into the medium. From the resulting suspension dilute cells to a concentration 1-2 X 10exp5 cells/ml into fresh medium in a new flasks. Keep culture below approximate density of 10exp6 cells/ml.

Subc*tion Ratio: A subc*tion ratio of 1:2 to 1:3 is recommended

Medium Renewal: 2 to 3 times per week

Subcultures are prepared by scraping the adherent cells into the medium, and diluting the resulting suspension into fresh medium in new flasks.

Doubling Time: 36 to 48 hrs

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2001

recommended serum:ATCC 30-2020

References: 23348: Ogura M, et al. Establishment of a novel human megakaryoblastic leukemia cell line, MEG- 01, with positive Philadelphia chromosome. Blood 66: 1384-1392, 1985. PubMed: 2998511

CRL-2021 MEG-01
















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