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人骨肉瘤细胞

简要描述:HTB-94 SW 1353 人骨肉瘤细胞,
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  • 产品型号:SW 1353
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  • 更新时间:2024-03-11
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HTB-94 SW 1353 人骨肉瘤细胞

ATCC® Number: HTB-94™ Price: $338.00

Designations: SW 1353 [SW 1353, SW-1353]

Depositors: A Leibovitz

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

HTB-94 SW 1353 人骨肉瘤细胞

Source: Organ: bone

Disease: chondrosarcoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Roche FuGENE® Transfection Reagents)

Antigen Expression: Blood type O-

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 12,13

D16S539: 11,12

D5S818: 10,11

D7S820: 9,11

THO1: 6,9

TPOX: 8,11

vWA: 16,17

Cytogenetic Analysis: hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident

Isoenzymes: AK-1, 1

ES-D, 2

G6PD, B

GLO-I, 2

PGM1, 1

PGM3, 2

Age: 72 years

Gender: female

Ethnicity: Caucasian

Comments: The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian.

The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics.

A frozen ampule at passage 12 was received at the ATCC in January, 1982.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subc*tion Ratio: A subc*tion ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020



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