咨询热线

15000266580

当前位置:首页 >产品中心>细胞库>人肿瘤细胞、癌细胞>SW 1353人骨肉瘤细胞

人骨肉瘤细胞

简要描述:HTB-94 SW 1353 人骨肉瘤细胞,
原代细胞|细胞系|细胞株|菌种;细胞库管理规范,
提供的细胞株背景清楚,提供参考文献和培养条件!

  • 产品型号:SW 1353
  • 厂商性质:生产厂家
  • 更新时间:2022-09-05
  • 访  问  量:899

产品分类

Product Category

相关文章

Related Articles

详细介绍

HTB-94 SW 1353 人骨肉瘤细胞

ATCC® Number: HTB-94™ Price: $338.00

Designations: SW 1353 [SW 1353, SW-1353]

Depositors: A Leibovitz

Biosafety Level: 1

Shipped: frozen

Medium & Serum: See Propagation

Growth Properties: adherent

Organism: Homo sapiens (human)

Morphology: fibroblast

HTB-94 SW 1353 人骨肉瘤细胞

Source: Organ: bone

Disease: chondrosarcoma

Permits/Forms: In addition to the MTA mentioned above, other ATCC and/or regulatory permits may be required for the transfer of this ATCC material. Anyone purchasing ATCC material is ultimay responsible for obtaining the permits. Please click here for information regarding the specific requirements for shipment to your location.


Applications: transfection host (Roche FuGENE® Transfection Reagents)

Antigen Expression: Blood type O-

DNA Profile (STR): Amelogenin: X

CSF1PO: 12

D13S317: 12,13

D16S539: 11,12

D5S818: 10,11

D7S820: 9,11

THO1: 6,9

TPOX: 8,11

vWA: 16,17

Cytogenetic Analysis: hyperdiploid 47, XX, +7; Except for the trisomic N7 no other chromosome markers are evident

Isoenzymes: AK-1, 1

ES-D, 2

G6PD, B

GLO-I, 2

PGM1, 1

PGM3, 2

Age: 72 years

Gender: female

Ethnicity: Caucasian

Comments: The SW 1353 cell line was initiated by A. Leibovitz at the Scott and White Clinic, Temple, Texas in 1977 from a primary grade II chondrosarcoma of the right humerus obtained from a 72 year old female Caucasian.

The initial culture medium was L-15 containing cortisone and insulin plus 10% fetal bovine serum and antibiotics.

A frozen ampule at passage 12 was received at the ATCC in January, 1982.

Propagation: ATCC complete growth medium: The base medium for this cell line is ATCC-formulated Leibovitz's L-15 Medium, Catalog No. 30-2008. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.

Temperature: 37.0°C

Subculturing: Protocol: Remove medium, and rinse with 0.25% trypsin, 0.03% EDTA solution. Remove the solution and add an additional 1 to 2 ml of trypsin-EDTA solution. Allow the flask to sit at room temperature (or at 37C) until the cells detach. Add fresh culture medium, aspirate and dispense into new culture flasks.

Subcultivation Ratio: A subcultivation ratio of 1:2 is recommended

Medium Renewal: 2 to 3 times per week

Preservation: Freeze medium: Complete growth medium, 95%; DMSO, 5%

Storage temperature: liquid nitrogen vapor temperature

Related Products: Recommended medium (without the additional supplements or serum described under ATCC Medium):ATCC 30-2008

recommended serum:ATCC 30-2020



HTB-94 SW 1353













产品咨询

留言框

  • 产品:

  • 您的单位:

  • 您的姓名:

  • 联系电话:

  • 常用邮箱:

  • 省份:

  • 详细地址:

  • 补充说明:

  • 验证码:

    请输入计算结果(填写阿拉伯数字),如:三加四=7
联系方式

邮箱:xiangfbio@163.com

地址:上海市虹口区赤峰路630弄南雁大厦

咨询热线

400-821-8510

(周一至周日9:00- 19:00)

在线咨询
  • 扫一扫 微信咨询

Copyright©2022 上海复祥生物科技有限公司 All Right Reserved    备案号:沪ICP备10013034号-2     sitemap.xml
技术支持:化工仪器网    管理登陆